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Phosphoinositide‐dependent kinase 1–associated glycolysis is regulated by miR‐409‐3p in clear cell renal cell carcinoma
Clear cell renal cell carcinoma (ccRCC) is the most popular kidney cancer in adults. Metabolic shift toward aerobic glycolysis is a fundamental factor for ccRCC therapy. MicroRNAs (miRNAs) are thought to be important regulators in ccRCC development and progression. Phosphoinositide‐dependent kinase...
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| Published in: | Journal of cellular biochemistry 2019-01, Vol.120 (1), p.126-134 |
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| creator | Wang, Yongjun He, Yanfa Bai, Hongzhong Dang, Yi Gao, Jiangyan Lv, Pei |
| description | Clear cell renal cell carcinoma (ccRCC) is the most popular kidney cancer in adults. Metabolic shift toward aerobic glycolysis is a fundamental factor for ccRCC therapy. MicroRNAs (miRNAs) are thought to be important regulators in ccRCC development and progression. Phosphoinositide‐dependent kinase 1 (PDK1) is required for metabolic activation; however, the role of PDK1‐induced glycolytic metabolism regulated by miRNAs is unclear in ccRCC. So, the purpose of the current study is to elucidate the underlying mechanism in ccRCC cell metabolism mediated by PDK1. Our results revealed that miR‐409‐3p inhibited glycolysis by regulating PDK1 expression in ccRCC cells. We also found that miR‐409‐3p was regulated by hypoxia. Our results indicated that PDK1 facilitated ccRCC cell glycolysis, regulated by miR‐409‐3p in hypoxia.
MiR‐409‐3p is a target gene of phosphoinositide‐dependent kinase 1 in clear cell renal cell carcinoma (ccRCC) cells and tissues, which is downregulated by hypoxia/hypoxia‐inducible factor 1‐α. MiR‐409‐3p decreases ccRCC cell survival ability and glycolysis. |
| doi_str_mv | 10.1002/jcb.27152 |
| format | article |
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MiR‐409‐3p is a target gene of phosphoinositide‐dependent kinase 1 in clear cell renal cell carcinoma (ccRCC) cells and tissues, which is downregulated by hypoxia/hypoxia‐inducible factor 1‐α. MiR‐409‐3p decreases ccRCC cell survival ability and glycolysis.</description><identifier>ISSN: 0730-2312</identifier><identifier>EISSN: 1097-4644</identifier><identifier>DOI: 10.1002/jcb.27152</identifier><identifier>PMID: 30218446</identifier><language>eng</language><publisher>United States: Wiley Subscription Services, Inc</publisher><subject>3-Phosphoinositide-Dependent Protein Kinases - genetics ; 3-Phosphoinositide-Dependent Protein Kinases - metabolism ; Adults ; Carcinoma, Renal Cell - metabolism ; Carcinoma, Renal Cell - pathology ; Carcinoma, Renal Cell - surgery ; Cell Hypoxia ; Cell Line, Tumor ; Cell Proliferation ; clear cell renal cell carcinoma ; Clear cell-type renal cell carcinoma ; Gene Expression Regulation, Neoplastic ; Gene Knockdown Techniques ; Glucose - metabolism ; Glycolysis ; Humans ; Hypoxia ; Hypoxia-Inducible Factor 1, alpha Subunit - metabolism ; Kidney cancer ; Kidney Neoplasms - metabolism ; Kidney Neoplasms - pathology ; Kidney Neoplasms - surgery ; Kidneys ; Metabolic activation ; Metabolic rate ; Metabolism ; MicroRNAs - chemistry ; MicroRNAs - metabolism ; miRNA ; miR‐409‐3p ; Molecular Mimicry - genetics ; Oxygen Consumption ; phosphoinositide‐dependent kinase 1 ; Regulators ; Transfection</subject><ispartof>Journal of cellular biochemistry, 2019-01, Vol.120 (1), p.126-134</ispartof><rights>2018 Wiley Periodicals, Inc.</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c3882-5a6a56edff80b53c00c4356e66e24cdc025fa3f4653d443bbe07ea8f9c58b19f3</citedby><cites>FETCH-LOGICAL-c3882-5a6a56edff80b53c00c4356e66e24cdc025fa3f4653d443bbe07ea8f9c58b19f3</cites><orcidid>0000-0002-1480-8700</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,780,784,27924,27925</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/30218446$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Wang, Yongjun</creatorcontrib><creatorcontrib>He, Yanfa</creatorcontrib><creatorcontrib>Bai, Hongzhong</creatorcontrib><creatorcontrib>Dang, Yi</creatorcontrib><creatorcontrib>Gao, Jiangyan</creatorcontrib><creatorcontrib>Lv, Pei</creatorcontrib><title>Phosphoinositide‐dependent kinase 1–associated glycolysis is regulated by miR‐409‐3p in clear cell renal cell carcinoma</title><title>Journal of cellular biochemistry</title><addtitle>J Cell Biochem</addtitle><description>Clear cell renal cell carcinoma (ccRCC) is the most popular kidney cancer in adults. Metabolic shift toward aerobic glycolysis is a fundamental factor for ccRCC therapy. MicroRNAs (miRNAs) are thought to be important regulators in ccRCC development and progression. Phosphoinositide‐dependent kinase 1 (PDK1) is required for metabolic activation; however, the role of PDK1‐induced glycolytic metabolism regulated by miRNAs is unclear in ccRCC. So, the purpose of the current study is to elucidate the underlying mechanism in ccRCC cell metabolism mediated by PDK1. Our results revealed that miR‐409‐3p inhibited glycolysis by regulating PDK1 expression in ccRCC cells. We also found that miR‐409‐3p was regulated by hypoxia. Our results indicated that PDK1 facilitated ccRCC cell glycolysis, regulated by miR‐409‐3p in hypoxia.
MiR‐409‐3p is a target gene of phosphoinositide‐dependent kinase 1 in clear cell renal cell carcinoma (ccRCC) cells and tissues, which is downregulated by hypoxia/hypoxia‐inducible factor 1‐α. MiR‐409‐3p decreases ccRCC cell survival ability and glycolysis.</description><subject>3-Phosphoinositide-Dependent Protein Kinases - genetics</subject><subject>3-Phosphoinositide-Dependent Protein Kinases - metabolism</subject><subject>Adults</subject><subject>Carcinoma, Renal Cell - metabolism</subject><subject>Carcinoma, Renal Cell - pathology</subject><subject>Carcinoma, Renal Cell - surgery</subject><subject>Cell Hypoxia</subject><subject>Cell Line, Tumor</subject><subject>Cell Proliferation</subject><subject>clear cell renal cell carcinoma</subject><subject>Clear cell-type renal cell carcinoma</subject><subject>Gene Expression Regulation, Neoplastic</subject><subject>Gene Knockdown Techniques</subject><subject>Glucose - metabolism</subject><subject>Glycolysis</subject><subject>Humans</subject><subject>Hypoxia</subject><subject>Hypoxia-Inducible Factor 1, alpha Subunit - metabolism</subject><subject>Kidney cancer</subject><subject>Kidney Neoplasms - metabolism</subject><subject>Kidney Neoplasms - pathology</subject><subject>Kidney Neoplasms - surgery</subject><subject>Kidneys</subject><subject>Metabolic activation</subject><subject>Metabolic rate</subject><subject>Metabolism</subject><subject>MicroRNAs - chemistry</subject><subject>MicroRNAs - metabolism</subject><subject>miRNA</subject><subject>miR‐409‐3p</subject><subject>Molecular Mimicry - genetics</subject><subject>Oxygen Consumption</subject><subject>phosphoinositide‐dependent kinase 1</subject><subject>Regulators</subject><subject>Transfection</subject><issn>0730-2312</issn><issn>1097-4644</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2019</creationdate><recordtype>article</recordtype><recordid>eNp1kd9K3UAQhxdpqUfbC1-gBHqjF9HZfznJZT2obRGUotdhs5nonm6ycfcEyZU-guAb-iTuMeqFUBh2luHjm4EfITsU9ikAO1jqap_NqWQbZEahmKciE-ITmcGcQ8o4ZZtkK4QlABQFZ1_IJgdGcyGyGbk7v3ahv3amc8GsTI1P9w819tjV2K2Sf6ZTARP6dP-oQnDaqBXWyZUdtbNjMCGJ5fFqsC_zakxa8zcKBBTx5X1iukRbVD7RaG0kO2Wnr1Zex5Wt-ko-N8oG_Pbat8nl8dHF4ld6enbye_HzNNU8z1kqVaZkhnXT5FBJrgG04HGQZciErjUw2SjeiEzyWgheVQhzVHlTaJlXtGj4NtmdvL13NwOGVdmasD5FdeiGUDIKEqQoeBHRHx_QpRt8PH1N8YxxISWL1N5Eae9C8NiUvTet8mNJoVynUsZUypdUIvv91ThULdbv5FsMETiYgFtjcfy_qfyzOJyUz9VbmpA</recordid><startdate>201901</startdate><enddate>201901</enddate><creator>Wang, Yongjun</creator><creator>He, Yanfa</creator><creator>Bai, Hongzhong</creator><creator>Dang, Yi</creator><creator>Gao, Jiangyan</creator><creator>Lv, Pei</creator><general>Wiley Subscription Services, Inc</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QL</scope><scope>7QP</scope><scope>7QR</scope><scope>7T7</scope><scope>7TK</scope><scope>7U9</scope><scope>8FD</scope><scope>C1K</scope><scope>FR3</scope><scope>H94</scope><scope>K9.</scope><scope>M7N</scope><scope>P64</scope><scope>7X8</scope><orcidid>https://orcid.org/0000-0002-1480-8700</orcidid></search><sort><creationdate>201901</creationdate><title>Phosphoinositide‐dependent kinase 1–associated glycolysis is regulated by miR‐409‐3p in clear cell renal cell carcinoma</title><author>Wang, Yongjun ; He, Yanfa ; Bai, Hongzhong ; Dang, Yi ; Gao, Jiangyan ; Lv, Pei</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c3882-5a6a56edff80b53c00c4356e66e24cdc025fa3f4653d443bbe07ea8f9c58b19f3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2019</creationdate><topic>3-Phosphoinositide-Dependent Protein Kinases - genetics</topic><topic>3-Phosphoinositide-Dependent Protein Kinases - metabolism</topic><topic>Adults</topic><topic>Carcinoma, Renal Cell - metabolism</topic><topic>Carcinoma, Renal Cell - pathology</topic><topic>Carcinoma, Renal Cell - surgery</topic><topic>Cell Hypoxia</topic><topic>Cell Line, Tumor</topic><topic>Cell Proliferation</topic><topic>clear cell renal cell carcinoma</topic><topic>Clear cell-type renal cell carcinoma</topic><topic>Gene Expression Regulation, Neoplastic</topic><topic>Gene Knockdown Techniques</topic><topic>Glucose - metabolism</topic><topic>Glycolysis</topic><topic>Humans</topic><topic>Hypoxia</topic><topic>Hypoxia-Inducible Factor 1, alpha Subunit - metabolism</topic><topic>Kidney cancer</topic><topic>Kidney Neoplasms - metabolism</topic><topic>Kidney Neoplasms - pathology</topic><topic>Kidney Neoplasms - surgery</topic><topic>Kidneys</topic><topic>Metabolic activation</topic><topic>Metabolic rate</topic><topic>Metabolism</topic><topic>MicroRNAs - chemistry</topic><topic>MicroRNAs - metabolism</topic><topic>miRNA</topic><topic>miR‐409‐3p</topic><topic>Molecular Mimicry - genetics</topic><topic>Oxygen Consumption</topic><topic>phosphoinositide‐dependent kinase 1</topic><topic>Regulators</topic><topic>Transfection</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Wang, Yongjun</creatorcontrib><creatorcontrib>He, Yanfa</creatorcontrib><creatorcontrib>Bai, Hongzhong</creatorcontrib><creatorcontrib>Dang, Yi</creatorcontrib><creatorcontrib>Gao, Jiangyan</creatorcontrib><creatorcontrib>Lv, Pei</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Calcium & Calcified Tissue Abstracts</collection><collection>Chemoreception Abstracts</collection><collection>Industrial and Applied Microbiology Abstracts (Microbiology A)</collection><collection>Neurosciences Abstracts</collection><collection>Virology and AIDS Abstracts</collection><collection>Technology Research Database</collection><collection>Environmental Sciences and Pollution Management</collection><collection>Engineering Research Database</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>Algology Mycology and Protozoology Abstracts (Microbiology C)</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Journal of cellular biochemistry</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Wang, Yongjun</au><au>He, Yanfa</au><au>Bai, Hongzhong</au><au>Dang, Yi</au><au>Gao, Jiangyan</au><au>Lv, Pei</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Phosphoinositide‐dependent kinase 1–associated glycolysis is regulated by miR‐409‐3p in clear cell renal cell carcinoma</atitle><jtitle>Journal of cellular biochemistry</jtitle><addtitle>J Cell Biochem</addtitle><date>2019-01</date><risdate>2019</risdate><volume>120</volume><issue>1</issue><spage>126</spage><epage>134</epage><pages>126-134</pages><issn>0730-2312</issn><eissn>1097-4644</eissn><abstract>Clear cell renal cell carcinoma (ccRCC) is the most popular kidney cancer in adults. Metabolic shift toward aerobic glycolysis is a fundamental factor for ccRCC therapy. MicroRNAs (miRNAs) are thought to be important regulators in ccRCC development and progression. Phosphoinositide‐dependent kinase 1 (PDK1) is required for metabolic activation; however, the role of PDK1‐induced glycolytic metabolism regulated by miRNAs is unclear in ccRCC. So, the purpose of the current study is to elucidate the underlying mechanism in ccRCC cell metabolism mediated by PDK1. Our results revealed that miR‐409‐3p inhibited glycolysis by regulating PDK1 expression in ccRCC cells. We also found that miR‐409‐3p was regulated by hypoxia. Our results indicated that PDK1 facilitated ccRCC cell glycolysis, regulated by miR‐409‐3p in hypoxia.
MiR‐409‐3p is a target gene of phosphoinositide‐dependent kinase 1 in clear cell renal cell carcinoma (ccRCC) cells and tissues, which is downregulated by hypoxia/hypoxia‐inducible factor 1‐α. MiR‐409‐3p decreases ccRCC cell survival ability and glycolysis.</abstract><cop>United States</cop><pub>Wiley Subscription Services, Inc</pub><pmid>30218446</pmid><doi>10.1002/jcb.27152</doi><tpages>9</tpages><orcidid>https://orcid.org/0000-0002-1480-8700</orcidid><oa>free_for_read</oa></addata></record> |
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| subjects | 3-Phosphoinositide-Dependent Protein Kinases - genetics 3-Phosphoinositide-Dependent Protein Kinases - metabolism Adults Carcinoma, Renal Cell - metabolism Carcinoma, Renal Cell - pathology Carcinoma, Renal Cell - surgery Cell Hypoxia Cell Line, Tumor Cell Proliferation clear cell renal cell carcinoma Clear cell-type renal cell carcinoma Gene Expression Regulation, Neoplastic Gene Knockdown Techniques Glucose - metabolism Glycolysis Humans Hypoxia Hypoxia-Inducible Factor 1, alpha Subunit - metabolism Kidney cancer Kidney Neoplasms - metabolism Kidney Neoplasms - pathology Kidney Neoplasms - surgery Kidneys Metabolic activation Metabolic rate Metabolism MicroRNAs - chemistry MicroRNAs - metabolism miRNA miR‐409‐3p Molecular Mimicry - genetics Oxygen Consumption phosphoinositide‐dependent kinase 1 Regulators Transfection |
| title | Phosphoinositide‐dependent kinase 1–associated glycolysis is regulated by miR‐409‐3p in clear cell renal cell carcinoma |
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