Novel lytic bacteriophages of Klebsiella oxytoca ABG-IAUF-1 as the potential agents for mastitis phage therapy

Mastitis is an inflammation of the mammary gland that occurs when pathogenic microorganisms enter the udder. Even though tremendous advancements in veterinary diagnosis and therapeutics, mastitis is still the most frequent and costly disease of dairy herds overall the world. The purpose of this rese...

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Bibliographic Details
Published in:FEMS microbiology letters 2018-10, Vol.365 (20), p.1
Main Authors: Amiri Fahliyani, Sara, Beheshti-Maal, Keivan, Ghandehari, Fereshteh
Format: Article
Language:English
Subjects:
Analysis
Bacteria
Bacteriophages
Biological control
Biotechnology
Care and treatment
Cattle
Dairy cattle
Diseases
Electron microscopy
Food contamination
Health aspects
Inflammation
Klebsiella
Klebsiella oxytoca
Mammary gland
Mammary glands
Mastitis
Methods
Microbiology
Microorganisms
Microscopy
Milk
Novels
Pathogenic microorganisms
Phage therapy
Phages
Physiological aspects
RNA
rRNA 16S
Therapeutics
Transmission electron microscopy
Udder
Veterinary research
Wastewater
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Summary:Mastitis is an inflammation of the mammary gland that occurs when pathogenic microorganisms enter the udder. Even though tremendous advancements in veterinary diagnosis and therapeutics, mastitis is still the most frequent and costly disease of dairy herds overall the world. The purpose of this research was to isolate and identify the lytic phages as a potential method for biological control of bovine mastitis. In this study Klebsiella oxytoca was isolated from contaminated milk samples of Isfahan dairy herds, Isfahan, Iran and characterized as K. oxytoca ABG-IAUF-1 and its 16s-rRNA sequence was deposited in GenBank under the accession numbers of MF175803.1. Then, the four novel specific lytic bacteriophages of K. oxytoca ABG-IAUF-1 from Isfahan public wastewater were isolated and identified. The results of transmission electron microscopy indicated that theses isolated phages were related to Myoviridae and Podoviridae families of bacteriophages. Also the analysis of the growth curve of K. oxytoca ABG-IAUF-1 before and after treatment with lytic phage showed the 97% success rate of the phages in preventing of bacterial growth. This is the first report indicating the use of bacteriophages as the potential agents for eliminating the pathogenic bacteria responsible for bovine mastitis in Iran. The applications of these lytic phages could be an asset for biocontrolling of pathogenic agents in medical and veterinary biotechnology.
ISSN:1574-6968
0378-1097
1574-6968
DOI:10.1093/femsle/fny223