DNA staining with the fluorochromes EtBr, DAPI and YOYO-1 in the comet assay with tobacco plants after treatment with ethyl methanesulphonate, hyperthermia and DNase-I

We applied the alkaline version of the single-cell gel electrophoresis (comet) assay to roots and leaves of tobacco ( Nicotiana tabacum var. xanthi) seedlings or isolated leaf nuclei treated with: (1) the alkylating agent ethyl methanesulphonate, (2) necrotic heat treatments at 50 °C, and (3) DNase-...

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Bibliographic Details
Published in:Mutation research. Genetic toxicology and environmental mutagenesis 2006-06, Vol.605 (1), p.17-21
Main Authors: Gichner, Tomáš, Mukherjee, Anita, Velemínský, Jiří
Format: Article
Language:English
Subjects:
Biological and medical sciences
DNA migration
Fundamental and applied biological sciences. Psychology
Genetics of eukaryotes. Biological and molecular evolution
Medical sciences
Nicotiana tabacum
Single-cell gel electrophoresis
Toxicology
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Summary:We applied the alkaline version of the single-cell gel electrophoresis (comet) assay to roots and leaves of tobacco ( Nicotiana tabacum var. xanthi) seedlings or isolated leaf nuclei treated with: (1) the alkylating agent ethyl methanesulphonate, (2) necrotic heat treatments at 50 °C, and (3) DNase-I. All three treatments induced a dose-dependent increase in DNA migration, expressed as percentage of tail DNA. A comparison of the fluorochrome DNA dyes ethidium bromide, DAPI and YOYO-1 demonstrated that for the alkaline version of the comet assay in plants, the commonly used fluorescent dye ethidium bromide can be used with the same efficiency as DAPI or YOYO-1.
ISSN:1383-5718
1879-3592
DOI:10.1016/j.mrgentox.2006.01.005