Determination of deoxynivalenol in cereals by HPLC-UV

A simple method for determination of deoxynivalenol (DON) in cereal samples is described. DON was extracted with methanol, the solvent evaporated, and the residue redissolved with water. This extract was purified on immunoaffinity columns. DON was determined by HPLC with UV-detection. The limits of...

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Bibliographic Details
Published in:Mycotoxin research 2003-03, Vol.19 (1), p.31-34
Main Authors: Czerwiecki, L, Wilczyńska, G
Format: Article
Language:English
Subjects:
Cereals
Deoxynivalenol
Food contamination & poisoning
High-performance liquid chromatography
Liquid chromatography
Methanol
Toxicology
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Description
Summary:A simple method for determination of deoxynivalenol (DON) in cereal samples is described. DON was extracted with methanol, the solvent evaporated, and the residue redissolved with water. This extract was purified on immunoaffinity columns. DON was determined by HPLC with UV-detection. The limits of detection (LOD) and quantification (LOQ) were 10 and 50 µg/kg, respectively.
ISSN:0178-7888
1867-1632
DOI:10.1007/BF02940088