Simple and efficient cell disruption of extremely small quantities of mycelium of phytopathogenic mycotoxin-producing moulds for quantitative extraction of genomic DNA

DNA was efficiently and quantitatively isolated from extremely small quantities of mycelia (0.1-10 mg) of different phytopathogenic moulds by grinding freeze-dried mycelia with glass beads and then using a commercial DNA extraction kit. The efficiency of disruption of the mycelia and the quantitativ...

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Bibliographic Details
Published in:Mycotoxin research 2005-12, Vol.21 (4), p.213-217
Main Authors: Saß, V, Milles, J, Krämer, J, Prange, A
Format: Article
Language:English
Subjects:
Cell disruption
Deoxyribonucleic acid
Disruption
DNA
Glass beads
Mycelia
Mycotoxins
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Summary:DNA was efficiently and quantitatively isolated from extremely small quantities of mycelia (0.1-10 mg) of different phytopathogenic moulds by grinding freeze-dried mycelia with glass beads and then using a commercial DNA extraction kit. The efficiency of disruption of the mycelia and the quantitative DNA extraction was proved by microscopy and the quantification of isolated DNA by real time PCR.
ISSN:0178-7888
1867-1632
DOI:10.1007/BF02957580