Double immunization strategy with a BoHV-4-vectorialized secreted chimeric peptide BVDV-E2/BoHV-1-gD

• Published in: Vaccine 2008-11, Vol.26 (48), p.6031-6042
• Main Authors: Donofrio, G, Sartori, C, Franceschi, V, Capocefalo, A, Cavirani, S, Taddei, S, Flammini, C.F
• Format: Article
• Language: English
• Subjects: Abortion; Allergy and Immunology; Animals; Antigens; Applied microbiology; Bacterial infections; Biological and medical sciences; Blotting, Western; Bovine herpesvirus 1; Bovine herpesvirus 4; Bovine viral diarrhea virus; Bovine Virus Diarrhea-Mucosal Disease - immunology; Bovine Virus Diarrhea-Mucosal Disease - prevention & control; Cattle; Cells, Cultured; Chimeric peptide; Deoxyribonucleic acid; Diarrhea; Diarrhea Virus 1, Bovine Viral - genetics; Diarrhea Virus 1, Bovine Viral - immunology; Disease; DNA; DNA, Viral - genetics; DNA, Viral - isolation & purification; Fundamental and applied biological sciences. Psychology; Genetic Vectors; Genomes; Herpesvirus 1, Bovine - immunology; Herpesvirus 4, Bovine - genetics; Herpesvirus 4, Bovine - immunology; Immunization; Immunization - methods; In Situ Hybridization; Infections; Laboratory animals; Luciferases - genetics; Microbiology; Milk; Mutagenesis, Insertional; Mutant Chimeric Proteins - genetics; Mutant Chimeric Proteins - immunology; Phenols; Plasmids - genetics; Rabbits; Recombinant Fusion Proteins - genetics; Recombinant Fusion Proteins - immunology; Reverse Transcriptase Polymerase Chain Reaction; RNA, Viral - genetics; RNA, Viral - isolation & purification; Vaccines, antisera, therapeutical immunoglobulins and monoclonal antibodies (general aspects); Viral Envelope Proteins - genetics; Viral Envelope Proteins - immunology; Viral Plaque Assay; Viral Proteins - genetics; Viral Proteins - immunology; Viral vector
• ISSN: 0264-410X; 1873-2518
• DOI: 10.1016/j.vaccine.2008.09.023

Abstract A bovine herpesvirus 4 was isolated from the milk cell fraction of a healthy cow and his full genome cloned as a bacterial artificial chromosome. So cloned viral genome was used as a vector platform to deliver in vitro and in vivo an optimized secreted chimeric peptide obtained by the fusion of the bovine viral diarrhoea virus glycoprotein E2 ectodomain with the bovine herpesvirus 1 glycoprotein D ectodomain. Recombinant virus infected cells robustly expressed and secreted the chimeric peptide into the culture medium and inoculated animals with the recombinant virus successfully responded toward antigens, gE2 and gD. Thus, this work has implications for the development of safe and effective polyvalent vaccines.