Membrane attack induced by HlyA, a pore-forming toxin of Vibrio cholerae

Determining the activity of purified toxins has generally provided the basis for establishing their role in the host- pathogen relationship. The bacterial genus Vibrio pro duces a number of exotoxins in addition to cholera toxin, including haemolysin A (HIyA; Vibrio cholerae) and thermostable direct...

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Bibliographic Details
Published in:Human & experimental toxicology 1997-02, Vol.16 (2), p.101-105
Main Authors: Huntley, James S, Sathyamoorthy, Venugopal, Hall, Robert H, Hall, Andrew C
Format: Article
Language:English
Subjects:
Adult
Bacterial Proteins
Bacterial Toxins
Cell Membrane Permeability
Dose-Response Relationship, Drug
Erythrocytes - drug effects
Erythrocytes - metabolism
Hemolysin Proteins - chemistry
Hemolysin Proteins - toxicity
Hemolysis
Humans
Potassium - metabolism
Rubidium
Sodium - metabolism
Vibrio cholerae
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Summary:Determining the activity of purified toxins has generally provided the basis for establishing their role in the host- pathogen relationship. The bacterial genus Vibrio pro duces a number of exotoxins in addition to cholera toxin, including haemolysin A (HIyA; Vibrio cholerae) and thermostable direct haemolysin (TDH; Vibrio parahae molyticus), both of which possess membrane-targeting cytolytic activity. The action of HlyA has been analyzed using protocols previously applied to TDH: lysis and flux experiments on human erythrocytes showed that HlyA similarly causes lysis after cell swelling (by colloid osmosis) due to an elevation of cation permeability. However, kinetic measurements of flux, haemolysis and cation selectivity showed that HlyA and TDH form pores with distinct and characteristic features.
ISSN:0960-3271
1477-0903
DOI:10.1177/096032719701600205