Modelling and simulation of a transketolase mediated reaction: Sensitivity analysis of kinetic parameters

In this paper we have used a proposed mathematical model, describing the carbon–carbon bond formation reaction between β-hydroxypyruvate and glycolaldehyde to synthesise l-erythrulose, catalysed by the enzyme transketolase, for the analysis of the sensitivity of the process to its kinetic parameters...

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Bibliographic Details
Published in:Biochemical engineering journal 2009-12, Vol.47 (1), p.1-9
Main Authors: Sayar, N.A., Chen, B.H., Lye, G.J., Woodley, J.M.
Format: Article
Language:English
Subjects:
Biocatalysis
Bioconversions. Hemisynthesis
Biological and medical sciences
Biotechnology
Enzyme
Fundamental and applied biological sciences. Psychology
Kinetics
Mathematical modelling
Methods. Procedures. Technologies
Sensitivity analysis
Transketolase
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Summary:In this paper we have used a proposed mathematical model, describing the carbon–carbon bond formation reaction between β-hydroxypyruvate and glycolaldehyde to synthesise l-erythrulose, catalysed by the enzyme transketolase, for the analysis of the sensitivity of the process to its kinetic parameters. The model was validated with experimental data. As a conclusion, kinetic parameters with a possible positive impact on reaction performance were identified and assessed in relation to operating conditions. This resulted in the identification of suitable catalyst and process development targets.
ISSN:1369-703X
1873-295X
DOI:10.1016/j.bej.2009.02.011