Pretreatment with heat does not affect double-strand breaks DNA rejoining in Chlamydomonas reinhardtii

We aimed to clarify if heat pretreatment could protect Chlamydomonas reinhardtii cells from gamma rays DNA damaging action. It was studied whether: (1) heat pretreatment could accelerate DNA DSB rejoining; (2) chloroplast chaperones (HSP70B, HSP90C) could be involved in protection from radiation-ind...

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Bibliographic Details
Published in:Journal of thermal biology 2009-10, Vol.34 (7), p.332-336
Main Authors: Chankova, Stephka G., Yurina, Nadezhda P., Dimova, Evgeniya G., Ermohina, Olga V., Oleskina, Yulia P., Dimitrova, Maria T., Bryant, Peter E.
Format: Article
Language:English
Subjects:
Adaptive response
Chlamydomonas
Chlamydomonas reinhardtii
DNA DSB rejoining
Gamma rays
Heat-shock proteins
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Summary:We aimed to clarify if heat pretreatment could protect Chlamydomonas reinhardtii cells from gamma rays DNA damaging action. It was studied whether: (1) heat pretreatment could accelerate DNA DSB rejoining; (2) chloroplast chaperones (HSP70B, HSP90C) could be involved in protection from radiation-induced DNA DSB. It was obtained that heat pretreatment (37–42 °C) induced minor DNA DSB levels which might be insufficient as signals for DNA DSB repair induction. No correlation between chaperones overproduction and DNA DSB rejoining was shown. These are probably the first data that HSP70B and HSP90C do not protect DNA against radiation-induced damage in a plant model system.
ISSN:0306-4565
1879-0992
DOI:10.1016/j.jtherbio.2009.06.002