Protective Effect of Dexamethasone on Lipopolysaccharide-Induced Inhibition of Contractile Function of Isolated Lymphatic Vessels and Nodes

• Published in: Bulletin of experimental biology and medicine 2018-09, Vol.165 (5), p.602-605
• Main Authors: Lobov, G. I., Unt, D. V.
• Format: Article
• Language: English
• Subjects: Amidines - pharmacology; Animals; Anti-Inflammatory Agents - pharmacology; Benzylamines - pharmacology; Biomedical and Life Sciences; Biomedicine; Cattle; Cell Biology; Central nervous system depressants; COX-2 inhibitors; Cyclooxygenase 2 - genetics; Cyclooxygenase 2 - metabolism; Cyclooxygenase-2; Dexamethasone; Dexamethasone - pharmacology; Endothelial Cells - cytology; Endothelial Cells - drug effects; Endothelial Cells - enzymology; Gene Expression; Internal Medicine; Isoxazoles - pharmacology; Laboratory Medicine; Lipopolysaccharides; Lipopolysaccharides - antagonists & inhibitors; Lipopolysaccharides - pharmacology; Lymph Nodes - cytology; Lymph Nodes - drug effects; Lymph Nodes - enzymology; Lymphatic system; Lymphatic Vessels - cytology; Lymphatic Vessels - drug effects; Lymphatic Vessels - enzymology; Male; Mitogens; Muscle contraction; Muscle Contraction - drug effects; Muscle, Smooth - cytology; Muscle, Smooth - drug effects; Muscle, Smooth - enzymology; Myocytes, Smooth Muscle - cytology; Myocytes, Smooth Muscle - drug effects; Myocytes, Smooth Muscle - enzymology; Nitric oxide; Nitric Oxide Synthase Type II - genetics; Nitric Oxide Synthase Type II - metabolism; Nitric-oxide synthase; Nodes; Pathology; Smooth muscle; Steroids; Steroids (Organic compounds); Tissue Culture Techniques
• ISSN: 0007-4888; 1573-8221
• DOI: 10.1007/s10517-018-4222-7

LPS has an inhibitory effect on contractile activity of bovine mesenteric lymphatic vessels and nodes and causes a pronounced decrease in the tone and phase contractions. The selective inhibitor of inducible NO synthase, 1400W, and cyclooxygenase-2 selective inhibitor, dynastat, significantly attenuated the inhibitory effect of LPS. Dexamethasone interferes with the inhibitory effect of LPS on bovine lymphatic vessels and nodes. It was concluded that LPS stimulates expression of inducible NO synthase and cyclooxygenase-2 in endothelial and smooth muscle cells of lymphatic vessels and nodes. Dexamethasone has a pronounced protective effect on the contractile function of lymphatic vessels and nodes affected by LPS and suppresses the expression of inducible NO synthase and cyclooxygenase-2.