Oral MucoRice expressing double-mutant cholera toxin A and B subunits induces toxin-specific neutralising immunity

• Published in: Vaccine 2009-10, Vol.27 (43), p.5982-5988
• Main Authors: Yuki, Yoshikazu, Tokuhara, Daisuke, Nochi, Tomonori, Yasuda, Hiroshi, Mejima, Mio, Kurokawa, Shiho, Takahashi, Yuko, Kataoka, Nobuhiro, Nakanishi, Ushio, Hagiwara, Yukari, Fujihashi, Kohtaro, Takaiwa, Fumio, Kiyono, Hiroshi
• Format: Article
• Language: English
• Subjects: Administration, Oral; Allergy and Immunology; Animals; Antibodies, Bacterial - blood; Antibodies, Bacterial - immunology; Antibody Formation - immunology; Applied microbiology; Bacterial diseases; Bacterial Vaccines - administration & dosage; Bacterial Vaccines - biosynthesis; Bacterial Vaccines - immunology; Bacteriology; Biological and medical sciences; Cholera; Cholera Toxin - immunology; Cloning; Cold-chain-free; Deoxyribonucleic acid; DNA; Drug dosages; Fundamental and applied biological sciences. Psychology; Genes; Genetic engineering; Genomes; Glycerol; Human bacterial diseases; Immunoglobulin A - blood; Immunoglobulin A - immunology; Immunoglobulin G - blood; Immunoglobulin G - immunology; Infectious diseases; Laboratories; Medical sciences; Mice; Mice, Inbred BALB C; Microbiology; Miscellaneous; MucoRice; Mutant cholera toxin; Neutralization Tests; Oral vaccine; Oryza - genetics; Oryza - immunology; Plants, Genetically Modified - genetics; Plants, Genetically Modified - immunology; Proteins; R&D; Research & development; Rice-based vaccine; Scholarships & fellowships; Seeds; Toxins; Tropical bacterial diseases; Vaccines; Vaccines, antisera, therapeutical immunoglobulins and monoclonal antibodies (general aspects); Waterborne diseases
• ISSN: 0264-410X; 1873-2518
• DOI: 10.1016/j.vaccine.2009.07.071

Abstract Rice-expressed cholera toxin B (CTB) subunit is a cold-chain-free oral vaccine that effectively induces enterotoxin-neutralising immunity. We created another rice-based vaccine, MucoRice, expressing nontoxic double-mutant cholera toxin (dmCT) with CTA and CTB subunits. Western-blot analysis suggested that MucoRice-dmCT had the shape of a multicomponent vaccine. Oral administration of MucoRice-dmCT induced CTB- but not CTA-specific serum IgG and mucosal IgA antibodies, generating protective immunity against cholera toxin without inducing rice-protein-specific antibody responses. The potency of MucoRice-dmCT was equal to that of MucoRice-CTB vaccine. MucoRice has the potential to be used as a safe multicomponent vaccine expression system.