Therapeutic Use of Cloning: Osmangazi Turk Identical Embryonic Stem Cells and Embryonic Stem Cell Transfer to Diabetic Mice

• Published in: Journal of Health Science 2009, Vol.55(4), pp.503-515
• Main Authors: Gurer, Firdevs, Ozden, Hilmi, Muslumanoglu, Hamza, Baycu, Cengiz, Cilingir, Oguz, Hassa, Hikmet
• Format: Article
• Language: English
• Subjects: glucose level; identical embryonic stem cells; regenerative medicine; streptozatocin-diabetes; therapeutic use of mouse cloning; β cells
• ISSN: 1344-9702; 1347-5207
• DOI: 10.1248/jhs.55.503

There are important problems in the therapeutic use of cloning which increase with the more advanced differentiation of somatic cells. Problems include DNA reprogramming, tissue rejection, and chromatin remodeling. This study presents the first use in somatic cell nuclear transfer (SCNT) of tooth pulp cells containing progenitor cells that resemble the nonaging embryonic connective tissue in adults. Using tooth pulp cells in SCNT increased the first cleavage rates compared with adult somatic cells, leading to a higher rate of DNA reprogramming and increased production of an identical embryonic stem cells (ESCs) line. ESCs derived from tooth pulp cells using this method were observed to transfer into pancreatic β cells using transmission electron microscopy. These cells were named Osmangazi Turk Identical ESCs since this was the first use in SCNT of tooth pulp cells to demonstrate a decrease in glucose levels following administration of these cells. Furthermore, nonidentical ESC perform tissue repair by decreasing glucose levels; however, the renewed tissue was observed to undergo important alterations which affect its future. The prognosis of the therapy of mice with diabetes mellitus using Osmangazi Turk Identical ESC was good, with an 80% therapeutic efficiency. These outcomes are promising for regenerative medicine in the therapy of diabetes mellitus, and clarification of the importance of selecting appropriate adult somatic cells in deriving identical ESC lines is also a significant step.