Blood serum acute phase proteins and iron dynamics during acute phase response of Salmonella enterica serotype Dublin experimentally infected buffalo calves

• Published in: Veterinary immunology and immunopathology 2018-09, Vol.203, p.30-39
• Main Authors: Santana, André M., Silva, Daniela G., Thomas, Funmilola C., Bernardes, Priscila A., Pizauro, Lucas J.L., Santana, Clarissa H., Burchmore, Richard J.S., Eckersall, Peter D., Fagliari, José J.
• Format: Article
• Language: English
• Subjects: Acute-Phase Proteins - analysis; Animals; Buffaloes - blood; Buffaloes - immunology; Buffaloes - microbiology; Ceruloplasmin; Ceruloplasmin - analysis; Electrophoresis; Electrophoresis, Gel, Two-Dimensional - veterinary; Electrophoresis, Polyacrylamide Gel - veterinary; Fibrinogen; Fibrinogen - analysis; Haptoglobin; Haptoglobins - analysis; Iron - blood; Salmonella enterica - immunology; Salmonella Infections, Animal - blood; Salmonella Infections, Animal - immunology; Salmonella Infections, Animal - microbiology; Transferrin; Transferrin - analysis; Water buffalo
• ISSN: 0165-2427; 1873-2534
• DOI: 10.1016/j.vetimm.2018.07.014

•APP are potential candidates to be used as biomarkers during buffalo salmonellosis.•Haptoglobin acts as a major positive APP during S. Dublin infection in buffaloes.•Ceruloplasmin acts as a positive APP during S. Dublin infection in buffalo calves.•Fibrinogen acts as a positive APP during S. Dublin infection in buffalo calves.•Iron and transferrin decreased during Salmonella Dublin infection in buffaloes. The study aimed to evaluate clinical signs, blood serum acute phase proteins (APP) and iron dynamics during the acute phase response (APR) of Salmonella Dublin experimentally infected Murrah buffalo calves. Six buffalo calves constituted the control group (CNT) and six were orally inoculate with 108 CFU of S. Dublin (INF). Clinical evaluation was performed, rectal swabs to detect S. Dublin strains were collected and venous blood was sampled before and throughout seven days after inoculation. The APP fractions β-haptoglobin, α-haptoglobin, ceruloplasmin and transferrin were analyzed by 1-D and 2-D electrophoresis. Proteins were identified using LC/ESI-MS/MS and NCBI database. Plasma fibrinogen, serum iron and serum haptoglobin concentrations were measured. The inoculation of 108 CFU of S. Dublin was effective in inducing clinical signs of Salmonellosis, such as hyperthermia and diarrhea. 1-DE showed that β and α-haptoglobin increased 204% (p = 0.008) and 184% (p = 0.022) 48 h after inoculation (HAI), respectively, with highest concentrations 120 HAI (498% increased, p = 0.012; 431% increased, p = 0.011) and 168 HAI (492% increased, p = 0.019; 523% increased, p = 0.028). 2-DE showed that the expression of two spots, identified as β-haptoglobin, were increased 693% (p = 0.0006) and 580% (p = 0.0003) 168 HAI, respectively, while one spot, identified as α-haptoglobin, increased 714% (p = 0.040). Haptoglobin concentrations increased 1339% (p