Genome-wide identification and characterization of the CsSnRK2 family in Camellia sinensis

The sucrose nonfermenting 1 (SNF1)-related protein kinase 2 (SnRK2) genes play central roles in plant stress signal transduction. In this study, 8 SnRK2 genes were identified from the tea plant genome database and named CsSnRK2.1-8. Phylogenetic analysis showed that the CsSnRK2 genes were classifiab...

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Bibliographic Details
Published in:Plant physiology and biochemistry 2018-11, Vol.132, p.287-296
Main Authors: Zhang, Yong-Heng, Wan, Si-Qing, Wang, Wei-Dong, Chen, Jiang-Fei, Huang, Lin-Li, Duan, Meng-Sha, Yu, You-Ben
Format: Article
Language:English
Subjects:
Abiotic stress
Camellia sinensis
Expression pattern
SnRK2 genes
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Summary:The sucrose nonfermenting 1 (SNF1)-related protein kinase 2 (SnRK2) genes play central roles in plant stress signal transduction. In this study, 8 SnRK2 genes were identified from the tea plant genome database and named CsSnRK2.1-8. Phylogenetic analysis showed that the CsSnRK2 genes were classifiable into three groups, similar to those of Arabidopsis thaliana, Oryza sativa and maize. The coding sequences (CDSs) of all CsSnRK2s were separated by eight introns, and their exon-intron organizations exhibited high similarity to those of other plants. The fluorescence of GFP fused with CsSnRK2.3 was detected in only the cytoplasm, while the rest of the proteins showed GFP signal in both the nucleus and the cytoplasm. The results of the expression patterns of the CsSnRK2 genes showed that CsSnRK2s were differentially induced by salt, polyethylene glycol (PEG) and abscisic acid (ABA) stress. Interestingly, The expression of CsSnRK2.3 was inhibited by ABA, suggesting the complicated roles of CsSnRK2s in the ABA signal transduction pathway. Some CsSnRK2 gene pairs showed significant expression change correlations under stresses, indicating that CsSnRK2s might exhibit synergistic effects of signal regulation in response to various stresses. In summary, this comprehensive analysis will facilitate further studies of the SnRK2 family of Camellia sinensis and provide useful information for the functional validation of CsSnRK2s. •We identified and characterized 8 CsSnRK2 genes and explored the expression under abiotic stresses.•We explored the subcellular localizations of CsSnRK2s.
ISSN:0981-9428
1873-2690
DOI:10.1016/j.plaphy.2018.09.021