Residual detection of naproxen, methyltestosterone and 17α‐hydroxyprogesterone caproate in aquatic products by simple liquid–liquid extraction method coupled with liquid chromatography–tandem mass spectrometry

In the present study, we aimed to develop a reliable screening method based on liquid chromatography coupled with tandem mass spectrometry (LC–MS/MS) for the detection and quantification of naproxen, methyltestosterone and 17α‐hydroxyprogesterone caproate residues. The target analytes were extracted...

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Bibliographic Details
Published in:Biomedical chromatography 2019-01, Vol.33 (1), p.e4396-n/a
Main Authors: Zheng, Weijia, Yoo, Kyung‐Hee, Choi, Jeong‐Min, Park, Da‐Hee, Kim, Seong‐Kwan, Kang, Young‐Sun, Abd El‐Aty, A. M., Hacımüftüoğlu, Ahmet, Wang, Jing, Shim, Jae‐Han, Shin, Ho‐Chul
Format: Article
Language:English
Subjects:
17 alpha-Hydroxyprogesterone Caproate - analysis
17 alpha-Hydroxyprogesterone Caproate - isolation & purification
17α‐hydroxyprogesterone carproate
Animals
aquatic product
Chromatography, Liquid - methods
Drug Residues - analysis
Eels
Flatfishes
LC–MS/MS
Limit of Detection
Linear Models
Liquid-Liquid Extraction - methods
methyltestosterone
Methyltestosterone - analysis
Methyltestosterone - isolation & purification
naproxen
Naproxen - analysis
Naproxen - isolation & purification
Penaeidae
Reproducibility of Results
residue
Seafood - analysis
Tandem Mass Spectrometry - methods
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Summary:In the present study, we aimed to develop a reliable screening method based on liquid chromatography coupled with tandem mass spectrometry (LC–MS/MS) for the detection and quantification of naproxen, methyltestosterone and 17α‐hydroxyprogesterone caproate residues. The target analytes were extracted from samples of eel, flatfish and shrimp using acetonitrile with 1% acetic acid, followed by liquid–liquid purification with n‐hexane. Chromatographic separation was achieved on a reversed‐phase analytical column using 0.1% formic acid containing 10 mm ammonium formate in distilled water (A) and methanol (B) as mobile phases. All the matrix‐matched calibration curves were linear (R2 ≥ 0.99) over the concentration range of the tested analytes. Recovery at three spiking levels (0.005, 0.01 and 0.02 mg/kg) ranged from 68 to 117% with intra‐ and inter‐day precisions
ISSN:0269-3879
1099-0801
DOI:10.1002/bmc.4396