Novel method for mass producing genetically sterile fish from surrogate broodstock via spermatogonial transplantation

A stable system for producing sterile domesticated fish is required to prevent genetic contamination to native populations caused by aquaculture escapees. The objective of this study was to develop a system to mass produce stock for aquaculture that is genetically sterile by surrogate broodstock via...

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Bibliographic Details
Published in:Biology of reproduction 2019-02, Vol.100 (2), p.535-546
Main Authors: Nagasawa, Kazue, Ishida, Mariko, Octavera, Anna, Kusano, Kazunari, Kezuka, Fumi, Kitano, Takeshi, Yoshiura, Yasutoshi, Yoshizaki, Goro
Format: Article
Language:English
Subjects:
Animals
Aquaculture
Aquaculture industry
Aromatase Inhibitors - pharmacology
Female
Females
Fish breeding
Follicle-stimulating hormone
Food contamination
fshr mutants
Gene Deletion
Gene Expression Regulation - drug effects
Gene Expression Regulation - genetics
Genetic research
Genotype
germline stem cells
Hormones
Hybridization, Genetic
Infertility - genetics
Infertility - veterinary
Male
Methods
Oryzias - genetics
Oryzias - physiology
Physiological aspects
Pituitary hormones
Receptors, FSH - genetics
Reproductive health
Reproductive technology
Sperm
Spermatogonia - physiology
spermatogonial transplantation
Stem cell transplantation
Stem cells
sterile fish production
Surgery
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Summary:A stable system for producing sterile domesticated fish is required to prevent genetic contamination to native populations caused by aquaculture escapees. The objective of this study was to develop a system to mass produce stock for aquaculture that is genetically sterile by surrogate broodstock via spermatogonial transplantation (SGTP).We previously discovered that female medaka carrying mutations on the follicle-stimulating hormone receptor (fshr) gene become sterile. In this study, we demonstrated that sterile hybrid recipient females that received spermatogonia isolated from sex-reversed XX males (fshr (–/–)) recovered their fertility and produced only donorderived fshr (–) X eggs. Natural mating between these females and fshr (–/–) sex-reversed XX males successfully produced large numbers of sterile fshr (–/–) female offspring. In conclusion, we established a new strategy for efficient mass production of sterile fish. This system can be applied to any aquaculture species for which SGTP and methods for producing sterile recipients can be established. Summary Sentence Novel method for mass producing genetically sterile fish from surrogate broodstock was established via transplantation of spermatogonia isolated from follicle-stimulating hormone receptor mutants
ISSN:0006-3363
1529-7268
DOI:10.1093/biolre/ioy204