Isolation of Pseudomonas stutzeri in wastewater of catfish fish-ponds in the Mekong Delta and its application for wastewater treatment

The aim of this study was to explore the potential for reducing soluble N load in fishpond wastewater using naturally occurring denitrifying bacteria. Twenty-seven isolates were selected from in wastewater (liquid/solid) of catfish-ponds located along the Tien river, in the Mekong Delta, Vietnam in...

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Bibliographic Details
Published in:Bioresource technology 2009-08, Vol.100 (16), p.3787-3791
Main Authors: Diep, Cao Ngoc, Cam, Pham My, Vung, Nguyen Hoai, Lai, To Thi, My, Nguyen Thi Xuan
Format: Article
Language:English
Subjects:
Ammonia
Applied sciences
Aquaculture
Biological and medical sciences
Brackish
catfish
denitrification
denitrifying bacteria
DNA Primers - genetics
Exact sciences and technology
fish culture
fish farms
fish ponds
Fishpond
Freshwater
freshwater aquaculture
freshwater fish
Fundamental and applied biological sciences. Psychology
nitrates
Nitrogen - metabolism
nitrogen oxides
Pangasianodon hypophthalmus
Pangasiidae
Pollution
Polymerase Chain Reaction
Pseudomonas stutzeri
Pseudomonas stutzeri - genetics
Pseudomonas stutzeri - isolation & purification
Pseudomonas stutzeri - metabolism
Rivers
RNA, Ribosomal, 16S - genetics
Siluriformes
Vietnam
Waste Disposal, Fluid - methods
Wastewater
Wastewater treatment
Wastewaters
Water Pollutants, Chemical - metabolism
Water Purification - methods
Water treatment and pollution
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Summary:The aim of this study was to explore the potential for reducing soluble N load in fishpond wastewater using naturally occurring denitrifying bacteria. Twenty-seven isolates were selected from in wastewater (liquid/solid) of catfish-ponds located along the Tien river, in the Mekong Delta, Vietnam in SW-LB medium (artificial seawater Luria-Britani medium) supplemented with 10 mM NH 4 and NO 3 and twenty-five isolates were identified as Pseudomonas stutzeri based on similarity of PCR-16S rRNA using universal primers and specific primers. Four isolates were effective in lowering soluble N (NH 4, NO 2 and NO 3) levels in fishpond water from 10 mg/L to negligible amounts after four days. Further experiments are underway to determine the fate of N lost from solution and the relative activity of ammonia oxidation, and nitrite and nitrate reduction by P. stutzeri isolates.
ISSN:0960-8524
1873-2976
DOI:10.1016/j.biortech.2009.02.021