Differential regulation of vimentin mRNA by 12- O-tetradecanoylphorbol 13-acetate and all-trans-retinoic acid correlates with motility of Hep 3B human hepatocellular carcinoma cells

Vimentin is a growth-related gene and often expressed when epithelial cells are stimulated to proliferate by growth factors. In cancer, vimentin expression is associated with a dedifferentiated malignant phenotype, increased motility, invasive ability and poor prognosis. We studied the regulation of...

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Bibliographic Details
Published in:Cancer letters 2004-01, Vol.203 (1), p.99-105
Main Authors: Yoon, Wan-Hee, Song, In-Sang, Lee, Byung-Hak, Jung, Yeon-Ju, Kim, Tae-Dong, Li, Ge, Lee, Taek-Gu, Park, Hae-Duck, Lim, Kyu, Hwang, Byung-Doo
Format: Article
Language:English
Subjects:
Breast cancer
Carcinoma, Hepatocellular - genetics
Carcinoma, Hepatocellular - pathology
Cell culture
Cell growth
Cell Movement - drug effects
Differential regulation
Dose-Response Relationship, Drug
Enzymes
Gene Expression Regulation, Neoplastic
Hep 3B cells
Humans
Hybridization
Invasion
Kinases
Liver cancer
Liver Neoplasms - genetics
Liver Neoplasms - pathology
Matrix Metalloproteinases - metabolism
Motility
Neoplasm Metastasis
RNA, Messenger - metabolism
Tetradecanoylphorbol Acetate - pharmacology
Time Factors
Tretinoin - pharmacology
Tumor Cells, Cultured
Urokinase-Type Plasminogen Activator - metabolism
Vimentin - genetics
Vimentin - metabolism
Vimentin mRNA
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Summary:Vimentin is a growth-related gene and often expressed when epithelial cells are stimulated to proliferate by growth factors. In cancer, vimentin expression is associated with a dedifferentiated malignant phenotype, increased motility, invasive ability and poor prognosis. We studied the regulation of vimentin mRNA and multistep invasion processes following treatment of 12- O-tetradecanoylphorbol 13-acetate (TPA) and all-trans-retinoic acid (RA) in Hep 3B hepatocellular carcinoma cells. TPA showed marked induction of vimentin mRNA, while RA decreased the mRNA level. TPA or RA did not affect cell proliferation, cell-matrix protein adhesion, and matrix metalloproteinases and urokinase plasminogen activator activities. In vitro invasion ability was significantly increased or decreased with TPA or RA treatment, paralleled to the in vitro motile activity, respectively. These findings suggest that TPA and RA could modulate the invasive potential of Hep 3B cells by altering cellular motility related to differential regulation of vimentin mRNA.
ISSN:0304-3835
1872-7980
DOI:10.1016/j.canlet.2003.08.004