Comparing the in vitro effects of MGO™ Manuka honey and tea tree oil on ocular Demodex viability

•In vitro anti-parasitic efficacy study of 52 Demodex mites.•Mites were randomised to cyclodextrin-complexed and uncomplexed Manuka honey, tea tree oil, or no treatment application.•Mite viability was assessed for 240 min following treatment application.•Cyclodextrin-complexed Manuka honey demonstra...

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Published in:Contact lens & anterior eye 2018-12, Vol.41 (6), p.527-530
Main Authors: Frame, Katie, Cheung, Isabella M.Y., Wang, Michael T.M., Turnbull, Philip R., Watters, Grant A., Craig, Jennifer P.
Format: Article
Language:English
Subjects:
Blepharitis
Demodex
Eyelid cleanser
Eyelid hygiene
Manuka honey
Tea tree oil
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description •In vitro anti-parasitic efficacy study of 52 Demodex mites.•Mites were randomised to cyclodextrin-complexed and uncomplexed Manuka honey, tea tree oil, or no treatment application.•Mite viability was assessed for 240 min following treatment application.•Cyclodextrin-complexed Manuka honey demonstrated comparable anti-demodectic efficacy to 50% tea tree oil (TTO).•Complexed honey with proven in vivo tolerability shows promise as possible alternative to TTO for demodex eradication. To compare the in vitro antiparasitic effects of MGO™ Manuka honey and tea tree oil against ocular Demodex. Fifty-two viable Demodex mites were acquired from the epilated eyelashes of 9 participants with blepharitis and symptomatic dry eye. Viable mites were randomised to one of five treatment groups: cyclodextrin-complexed and uncomplexed Manuka Honey, 100% and 50% tea tree oil, and no treatment. Following treatment application, mite viability was assessed for 240 min, based on limb and body movement and/or the development of a crenated/translucent appearance. Kaplan-Meier survival analysis was then performed. The log-rank test demonstrated a significant treatment effect on the survival distribution of Demodex mites (p 
doi_str_mv 10.1016/j.clae.2018.06.006
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To compare the in vitro antiparasitic effects of MGO™ Manuka honey and tea tree oil against ocular Demodex. Fifty-two viable Demodex mites were acquired from the epilated eyelashes of 9 participants with blepharitis and symptomatic dry eye. Viable mites were randomised to one of five treatment groups: cyclodextrin-complexed and uncomplexed Manuka Honey, 100% and 50% tea tree oil, and no treatment. Following treatment application, mite viability was assessed for 240 min, based on limb and body movement and/or the development of a crenated/translucent appearance. Kaplan-Meier survival analysis was then performed. The log-rank test demonstrated a significant treatment effect on the survival distribution of Demodex mites (p &lt; 0.001). Bonferroni-corrected post-hoc pairwise analysis showed that all treatments except for uncomplexed honey effected lower survival probabilities than the untreated group (all p &lt; 0.001). Among the four treatments, survival probabilities were lowest with 100% tea tree oil (all p &lt; 0.001), and highest with uncomplexed honey (all p ≤ 0.001). No difference was observed between complexed honey and 50% tea tree oil (p = 0.81). The in vitro efficacy of cyclodextrin-complexed Manuka honey was comparable with 50% tea tree oil, an established treatment for ocular Demodex. 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subjects Blepharitis
Demodex
Eyelid cleanser
Eyelid hygiene
Manuka honey
Tea tree oil
title Comparing the in vitro effects of MGO™ Manuka honey and tea tree oil on ocular Demodex viability
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