Ultrastructural and histological characteristics of the endometrium during early embryo development in mares

The aim of this study was to evaluate ultrastructural and histological changes in the endometrium on days 7, 10 and 13 post-ovulation in pregnant and cyclic mares. Mares were routinely examined by transrectal palpation and ultrasonographic examination of the reproductive tract until estrus was detec...

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Bibliographic Details
Published in:Theriogenology 2019-01, Vol.123, p.1-10
Main Authors: Camozzato, G.C., Martinez, M.N., Bastos, H.B.A., Fiala-Rechsteiner, S., Meikle, A., Jobim, M.I.M., Gregory, R.M., Mattos, R.C.
Format: Article
Language:English
Subjects:
Animals
Blood vessel
Ciliated cells
Embryonic Development
Endometrium - physiology
Endometrium - ultrastructure
Equine embryo
Female
Histotroph
Horses - embryology
Horses - physiology
Pre-implantation period
Pregnancy
Pregnancy, Animal - physiology
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Summary:The aim of this study was to evaluate ultrastructural and histological changes in the endometrium on days 7, 10 and 13 post-ovulation in pregnant and cyclic mares. Mares were routinely examined by transrectal palpation and ultrasonographic examination of the reproductive tract until estrus was detected. In the first cycle, endometrial biopsies from 30 cyclic mares (Cyclic group) were collected on days 7, 10 and 13 post-ovulation. In the second cycle, the same mares were bred by a fertile stallion. At days 7, 10 and 13 post-ovulation intrauterine biopsies were collected. Immediately after sample collection, the mare's uteri were flushed, and those mares with embryo recovery were assigned to the Pregnant group. From ovulation detection until day of uterine biopsy, blood samples to measure Progesterone concentrations were collected daily in cyclic and pregnant mares. A larger blood vessel caliber was observed in pregnant mares than in cyclic from day 7–13. On the 7th day of pregnancy a large loss of ciliated cells was evident in the group of pregnant mares in comparison with the Cyclic group and the superficial cells of the endometrium were more protruded, and a small amount of histotrophic material between the folds was observed. On the 10th day of pregnancy, the glandular histotrophic secretion and the secretion of luminal epithelium became more intense than the secretion of cyclic mares. On the 13th day of pregnancy, a very large amount of histotroph was observed within large glandular openings surrounded by ciliated cells. The concentrations of P4 were affected by day (P 
ISSN:0093-691X
1879-3231
DOI:10.1016/j.theriogenology.2018.09.018