Effects of Personal Short-Term Exposure to Ambient Ozone on Blood Pressure and Vascular Endothelial Function: A Mechanistic Study Based on DNA Methylation and Metabolomics

Short-term exposure to ambient ozone is associated with adverse cardiovascular effects, with inconsistent evidence on the molecular mechanisms. We conducted a longitudinal panel study among 43 college students in Shanghai to explore the effects of personal ozone exposure on blood pressure (BP), vasc...

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Bibliographic Details
Published in:Environmental science & technology 2018-11, Vol.52 (21), p.12774-12782
Main Authors: Xia, Yongjie, Niu, Yue, Cai, Jing, Lin, Zhijing, Liu, Cong, Li, Huichu, Chen, Chen, Song, Weimin, Zhao, Zhuohui, Chen, Renjie, Kan, Haidong
Format: Article
Language:English
Subjects:
Angiotensin
Biomarkers
Blood pressure
Cardiovascular disease
Correlation analysis
Deoxyribonucleic acid
DNA
DNA methylation
Emission measurements
Endothelin 1
Exposure
Human exposure
Lipid metabolism
Lipids
Loci
Metabolism
Metabolites
Metabolomics
Molecular modelling
Ozone
Peptidyl-dipeptidase A
Serum levels
Toxicity
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Summary:Short-term exposure to ambient ozone is associated with adverse cardiovascular effects, with inconsistent evidence on the molecular mechanisms. We conducted a longitudinal panel study among 43 college students in Shanghai to explore the effects of personal ozone exposure on blood pressure (BP), vascular endothelial function, and the potential molecular mechanisms. We measured real-time personal ozone exposure levels, serum angiotensin-converting enzyme (ACE) and endothelin-1 (ET-1), and locus-specific DNA methylation of ACE and EDN1 (coding ET-1). We used an untargeted metabolomic approach to explore potentially important metabolites. We applied linear mixed-effect models to examine the effects of ozone on the above biomarkers. An increase in 2 h-average ozone exposure was significantly associated with elevated levels of BP, ACE, and ET-1. ACE and EDN1 methylation decreased with ozone exposure, but the magnitude differed by genomic loci. Metabolomics analysis showed significant changes in serum lipid metabolites following ozone exposure that are involved in maintaining vascular endothelial function. Our findings suggested that acute exposure to ambient ozone can elevate serum levels of ACE and ET-1, decrease their DNA methylation, and alter the lipid metabolism, which may be partly responsible for the effects of ozone on BP and vascular endothelial function.
ISSN:0013-936X
1520-5851
DOI:10.1021/acs.est.8b03044