Extraction and determination of tricyclic antidepressants in real samples using air‐dispersed liquid–liquid microextraction prior to gas chromatography and flame ionization detection

In the present research, a novel microextraction method was used based on dispersive liquid–liquid microextraction using low‐density solvent for the determination of trace amounts of amitriptyline and doxepin as model compounds in human plasma and spring water samples before gas chromatography and f...

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Bibliographic Details
Published in:Journal of separation science 2018-12, Vol.41 (23), p.4340-4347
Main Authors: Mofazzeli, Farideh, Asaadi Shirvan, Homeira, Mohammadi, Fatemeh
Format: Article
Language:English
Subjects:
Air injection
amitriptyline
Analytical chemistry
Antidepressants
Blood plasma
Chromatography
Correlation coefficients
Dispersion
doxepin
Flame ionization
Gas chromatography
Ionization
Ions
microextraction
sample preparation
Solvents
Spring water
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Summary:In the present research, a novel microextraction method was used based on dispersive liquid–liquid microextraction using low‐density solvent for the determination of trace amounts of amitriptyline and doxepin as model compounds in human plasma and spring water samples before gas chromatography and flame ionization detection. The parameters influencing the extraction efficiencies including type of the extracting solvent, volume of the aqueous sample solution (donor phase), volume of the extraction solvent (acceptor phase), the number of air‐injection, and the effects of pH and ionic strength were optimized. Under the optimized conditions, the obtained enrichment factors were >80. By plotting peak areas of the sample solutions versus various concentrations of the analytes, calibration curves were obtained which show the linear ranges of 10–3000 ng/mL with correlation coefficients of >0.996. The precision of the method was calculated 91%.
ISSN:1615-9306
1615-9314
DOI:10.1002/jssc.201800359