Differential susceptibility to tetracycline, oxytetracycline and doxycycline of the calf pathogens Mannheimia haemolytica and Pasteurella multocida in three growth media

For clinical isolates of bovine Mannheimia haemolytica and Pasteurella multocida, this study reports minimum inhibitory concentration (MIC) differences for tetracycline, oxytetracycline and doxycycline between cation‐adjusted Mueller‐Hinton broth (CAMHB), foetal bovine serum (FBS) and Roswell Park M...

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Bibliographic Details
Published in:Journal of veterinary pharmacology and therapeutics 2019-01, Vol.42 (1), p.52-59
Main Authors: Mead, Andrew, Lees, Peter, Mitchell, John, Rycroft, Andrew, Standing, Joseph F., Toutain, Pierre‐Louis, Pelligand, Ludovic
Format: Article
Language:English
Subjects:
doxycycline
Mannheimia haemolytica
minimum inhibitory concentration
oxytetracycline
Pasteurella multocida
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Summary:For clinical isolates of bovine Mannheimia haemolytica and Pasteurella multocida, this study reports minimum inhibitory concentration (MIC) differences for tetracycline, oxytetracycline and doxycycline between cation‐adjusted Mueller‐Hinton broth (CAMHB), foetal bovine serum (FBS) and Roswell Park Memorial Institute (RPMI) medium. MICs were determined according to CLSI standards and additionally using five overlapping sets of twofold dilutions. Matrix effect: (a) free drug MICs and minimum bactericidal concentrations (MBC) for all drugs were significantly higher in FBS than in CAMHB for both pathogens (p 
ISSN:0140-7783
1365-2885
DOI:10.1111/jvp.12719