Simultaneous Transcriptome Analysis of Host and Pathogen Highlights the Interaction Between Brassica oleracea and Sclerotinia sclerotiorum

• Published in: Phytopathology 2019-04, Vol.109 (4), p.542-550
• Main Authors: Ding, Yijuan, Mei, Jiaqin, Chai, Yaru, Yu, Yang, Shao, Chaoguo, Wu, Qinan, Disi, Joseph Onwusemu, Li, Yuhua, Wan, Huafang, Qian, Wei
• Format: Article
• Language: English
• Subjects: Ascomycota - pathogenicity; Brassica - microbiology; Gene Expression Profiling; Plant Diseases; Transcriptome
• ISSN: 0031-949X; 1943-7684
• DOI: 10.1094/PHYTO-06-18-0204-R

White mold disease caused by Sclerotinia sclerotiorum is a devastating disease of Brassica crops. Here, we simultaneously assessed the transcriptome changes from lesions produced by S. sclerotiorum on disease-resistant (R) and -susceptible (S) B. oleracea pools bulked from a resistance-segregating F2 population. Virulence genes of S. sclerotiorum, including polygalacturonans, chitin synthase, secretory proteins, and oxalic acid biosynthesis, were significantly repressed in lesions of R B. oleracea at 12 h postinoculation (hpi) but exhibited similar expression patterns in R and S B. oleracea at 24 hpi. Resistant B. oleracea induced expression of receptors potentially to perceive Sclerotinia signals during 0 to 12 hpi and deployed complex strategies to suppress the pathogen establishment, including the quick accumulation of reactive oxygen species via activating Ca signaling and suppressing pathogen oxalic acid generation in S. sclerotiorum. In addition, cell wall degradation was inhibited in the resistant B. oleracea potentially to prevent the expansion of Sclerotinia hyphae. The transcriptome changes in S. sclerotiorum and host revealed that resistant B. oleracea produces strong responses against S. sclerotiorum during early infection.