Gold Nanoparticle Couples with Entropy-Driven Toehold-Mediated DNA Strand Displacement Reaction on Magnetic Beads: Toward Ultrasensitive Energy-Transfer-Based Photoelectrochemical Detection of miRNA-141 in Real Blood Sample

Highly stable circulating microRNAs (miRNAs) are currently recognized as a novel potential biomarker for clinical cancer diagnosis in the early stage. However, limited by its low concentration, high sequence similarity, as well as the numerous interferences in body fluids, detection of miRNA in whol...

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Bibliographic Details
Published in:Analytical chemistry (Washington) 2018-10, Vol.90 (20), p.11892-11898
Main Authors: Zhang, Nan, Shi, Xiao-Mei, Guo, Hong-Qian, Zhao, Xiao-Zhi, Zhao, Wei-Wei, Xu, Jing-Juan, Chen, Hong-Yuan
Format: Article
Language:English
Subjects:
Beads
Biomarkers
Blood
Blood circulation
Body fluids
Cancer
Chemistry
Deoxyribonucleic acid
Diagnosis
DNA
Entropy
Gold
Magnetic separation
miRNA
Nanoparticles
Prostate
Prostate cancer
Prostate carcinoma
Ribonucleic acid
RNA
Sensitivity analysis
Studies
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Summary:Highly stable circulating microRNAs (miRNAs) are currently recognized as a novel potential biomarker for clinical cancer diagnosis in the early stage. However, limited by its low concentration, high sequence similarity, as well as the numerous interferences in body fluids, detection of miRNA in whole blood with sufficient selectivity and sensitivity is still challenging. Herein, we reported the integration of entropy-driven toehold-mediated DNA strand displacement (ETSD) reaction with magnetic beads (MB) toward the energy-transfer-based photoelectrochemical (PEC) detection of the prostate carcinoma (PCa) biomarker miRNA-141 in a real blood sample. In this protocol, the ETSD reaction was divided into two steps, and cooperated with magnetic separation, target extraction and amplification could be realized in a single test and ultrasensitive detection of miRNA-141 could be achieved in undiluted whole blood sample. This work proposed a new solution for sensitive biomolecular detection in a complex biological milieu and exhibited great promise for future clinical cancer diagnosis.
ISSN:0003-2700
1520-6882
DOI:10.1021/acs.analchem.8b01966