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Sperm DNA integrity in Landrace and Duroc boar semen and its relationship to litter size
Contents The sperm chromatin structure assay is a method for assessment of sperm DNA fragmentation, a parameter reported to be negatively related to field fertility in several mammal species. This method calculates a DNA fragmentation index (DFI) whose high values indicate abnormal chromatin structu...
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| Published in: | Reproduction in domestic animals 2019-02, Vol.54 (2), p.160-166 |
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| container_title | Reproduction in domestic animals |
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| creator | Myromslien, Frøydis D. Tremoen, Nina H. Andersen‐Ranberg, Ina Fransplass, Ragnhild Stenseth, Else‐Berit Zeremichael, Teklu T. van Son, Maren Grindflek, Eli Gaustad, Ann Helen |
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The sperm chromatin structure assay is a method for assessment of sperm DNA fragmentation, a parameter reported to be negatively related to field fertility in several mammal species. This method calculates a DNA fragmentation index (DFI) whose high values indicate abnormal chromatin structure. In this study, running from March 2010 until June 2017, the aim was to assess sperm DFI in stored liquid extended semen from two different pig breeds, Norwegian Landrace (NL; n = 693) and Norwegian Duroc (ND; n = 655), and to evaluate the influence on total number of piglets born (TNB). There was a significantly higher median DFI (p |
| doi_str_mv | 10.1111/rda.13322 |
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The sperm chromatin structure assay is a method for assessment of sperm DNA fragmentation, a parameter reported to be negatively related to field fertility in several mammal species. This method calculates a DNA fragmentation index (DFI) whose high values indicate abnormal chromatin structure. In this study, running from March 2010 until June 2017, the aim was to assess sperm DFI in stored liquid extended semen from two different pig breeds, Norwegian Landrace (NL; n = 693) and Norwegian Duroc (ND; n = 655), and to evaluate the influence on total number of piglets born (TNB). There was a significantly higher median DFI (p < 0.0001) in ejaculates from the 478 ND boars compared to the 452 NL boars. Data from 19,496 NL litters and 3,877 ND litters of the same boars were retrieved. For either breed, sow herd (p < 0.0001), parity (p < 0.05) and DFI (p < 0.05) showed significant effects on TNB. The DFI was negatively correlated to TNB in both breeds. The boars with the 5% lowest TNB had a least square means DFI of 3.05% and 2.24% in NL and ND, respectively, compared to 1.67% and 1.23% for the boars with the 5% highest TNB (p < 0.01). The DFI and the motility of the same semen samples were negatively correlated (p < 0.0001), and the high and low TNB groups showed significant differences in motility. However, this difference could not be used for practical prediction of TNB group (92.1% vs. 89.7%; p = 0.0038 and 92.3% vs. 89.5%; p = 0.018; NL and ND, respectively). In conclusion, our results indicate that sperm DNA integrity in semen with good motility and morphology may be an additional prediction parameter for fertility in pigs.]]></description><identifier>ISSN: 0936-6768</identifier><identifier>EISSN: 1439-0531</identifier><identifier>DOI: 10.1111/rda.13322</identifier><identifier>PMID: 30168871</identifier><language>eng</language><publisher>Germany: Blackwell Publishing Ltd</publisher><subject>Acridine Orange ; Animals ; boar ; Breeding ; Chromatin ; Chromatin - chemistry ; Chromatin - drug effects ; Deoxyribonucleic acid ; DNA ; DNA fragmentation ; DNA Fragmentation - drug effects ; DNA integrity ; Female ; Fertility ; Flow Cytometry ; Fragmentation ; Hogs ; Integrity ; Litter Size ; Male ; Morphology ; Motility ; Parameters ; Parity ; Pregnancy ; Semen ; semen analysis ; Semen Analysis - veterinary ; Semen Preservation - veterinary ; Sperm ; sperm chromatin structure assay ; Spermatozoa - drug effects ; Spermatozoa - physiology ; Swine</subject><ispartof>Reproduction in domestic animals, 2019-02, Vol.54 (2), p.160-166</ispartof><rights>2018 Blackwell Verlag GmbH</rights><rights>2018 Blackwell Verlag GmbH.</rights><rights>Copyright © 2019 Blackwell Verlag GmbH</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c3532-bad9b0c699be9046d76d915279ddb234e8499f8d36729f4a52d45f93f5cbaedb3</citedby><cites>FETCH-LOGICAL-c3532-bad9b0c699be9046d76d915279ddb234e8499f8d36729f4a52d45f93f5cbaedb3</cites><orcidid>0000-0002-7873-734X</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,780,784,27924,27925</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/30168871$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Myromslien, Frøydis D.</creatorcontrib><creatorcontrib>Tremoen, Nina H.</creatorcontrib><creatorcontrib>Andersen‐Ranberg, Ina</creatorcontrib><creatorcontrib>Fransplass, Ragnhild</creatorcontrib><creatorcontrib>Stenseth, Else‐Berit</creatorcontrib><creatorcontrib>Zeremichael, Teklu T.</creatorcontrib><creatorcontrib>van Son, Maren</creatorcontrib><creatorcontrib>Grindflek, Eli</creatorcontrib><creatorcontrib>Gaustad, Ann Helen</creatorcontrib><title>Sperm DNA integrity in Landrace and Duroc boar semen and its relationship to litter size</title><title>Reproduction in domestic animals</title><addtitle>Reprod Domest Anim</addtitle><description><![CDATA[Contents
The sperm chromatin structure assay is a method for assessment of sperm DNA fragmentation, a parameter reported to be negatively related to field fertility in several mammal species. This method calculates a DNA fragmentation index (DFI) whose high values indicate abnormal chromatin structure. In this study, running from March 2010 until June 2017, the aim was to assess sperm DFI in stored liquid extended semen from two different pig breeds, Norwegian Landrace (NL; n = 693) and Norwegian Duroc (ND; n = 655), and to evaluate the influence on total number of piglets born (TNB). There was a significantly higher median DFI (p < 0.0001) in ejaculates from the 478 ND boars compared to the 452 NL boars. Data from 19,496 NL litters and 3,877 ND litters of the same boars were retrieved. For either breed, sow herd (p < 0.0001), parity (p < 0.05) and DFI (p < 0.05) showed significant effects on TNB. The DFI was negatively correlated to TNB in both breeds. The boars with the 5% lowest TNB had a least square means DFI of 3.05% and 2.24% in NL and ND, respectively, compared to 1.67% and 1.23% for the boars with the 5% highest TNB (p < 0.01). The DFI and the motility of the same semen samples were negatively correlated (p < 0.0001), and the high and low TNB groups showed significant differences in motility. However, this difference could not be used for practical prediction of TNB group (92.1% vs. 89.7%; p = 0.0038 and 92.3% vs. 89.5%; p = 0.018; NL and ND, respectively). In conclusion, our results indicate that sperm DNA integrity in semen with good motility and morphology may be an additional prediction parameter for fertility in pigs.]]></description><subject>Acridine Orange</subject><subject>Animals</subject><subject>boar</subject><subject>Breeding</subject><subject>Chromatin</subject><subject>Chromatin - chemistry</subject><subject>Chromatin - drug effects</subject><subject>Deoxyribonucleic acid</subject><subject>DNA</subject><subject>DNA fragmentation</subject><subject>DNA Fragmentation - drug effects</subject><subject>DNA integrity</subject><subject>Female</subject><subject>Fertility</subject><subject>Flow Cytometry</subject><subject>Fragmentation</subject><subject>Hogs</subject><subject>Integrity</subject><subject>Litter Size</subject><subject>Male</subject><subject>Morphology</subject><subject>Motility</subject><subject>Parameters</subject><subject>Parity</subject><subject>Pregnancy</subject><subject>Semen</subject><subject>semen analysis</subject><subject>Semen Analysis - veterinary</subject><subject>Semen Preservation - veterinary</subject><subject>Sperm</subject><subject>sperm chromatin structure assay</subject><subject>Spermatozoa - drug effects</subject><subject>Spermatozoa - physiology</subject><subject>Swine</subject><issn>0936-6768</issn><issn>1439-0531</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2019</creationdate><recordtype>article</recordtype><recordid>eNp10MtKxDAUBuAgijOOLnwBCbjRRWdyaZtmOcx4g0HBC7graXOqkd5MWmR8euN0dCGYzQmHj5_Dj9AxJVPq38xqNaWcM7aDxjTkMiARp7toTCSPg1jEyQgdOPdGCI0SIfbRiBMaJ4mgY_T80IKt8PJ2jk3dwYs13dr_8ErV2qocsJ942dsmx1mjLHZQQb1Zms5hC6XqTFO7V9PirsGl6TrwyHzCIdorVOngaDsn6Ony4nFxHazurm4W81WQ84izIFNaZiSPpcxAkjDWItaSRkxIrTPGQ0hCKYtE81gwWYQqYjqMCsmLKM8U6IxP0NmQ29rmvQfXpZVxOZSlqqHpXcooDQURvhVPT__Qt6a3tb_OK5FQyijhXp0PKreNcxaKtLWmUnadUpJ-1536utNN3d6ebBP7rAL9K3_69WA2gA9Twvr_pPR-OR8ivwCjgIf8</recordid><startdate>201902</startdate><enddate>201902</enddate><creator>Myromslien, Frøydis D.</creator><creator>Tremoen, Nina H.</creator><creator>Andersen‐Ranberg, Ina</creator><creator>Fransplass, Ragnhild</creator><creator>Stenseth, Else‐Berit</creator><creator>Zeremichael, Teklu T.</creator><creator>van Son, Maren</creator><creator>Grindflek, Eli</creator><creator>Gaustad, Ann Helen</creator><general>Blackwell Publishing Ltd</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QO</scope><scope>8FD</scope><scope>FR3</scope><scope>P64</scope><scope>7X8</scope><orcidid>https://orcid.org/0000-0002-7873-734X</orcidid></search><sort><creationdate>201902</creationdate><title>Sperm DNA integrity in Landrace and Duroc boar semen and its relationship to litter size</title><author>Myromslien, Frøydis D. ; Tremoen, Nina H. ; Andersen‐Ranberg, Ina ; Fransplass, Ragnhild ; Stenseth, Else‐Berit ; Zeremichael, Teklu T. ; van Son, Maren ; Grindflek, Eli ; Gaustad, Ann Helen</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c3532-bad9b0c699be9046d76d915279ddb234e8499f8d36729f4a52d45f93f5cbaedb3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2019</creationdate><topic>Acridine Orange</topic><topic>Animals</topic><topic>boar</topic><topic>Breeding</topic><topic>Chromatin</topic><topic>Chromatin - chemistry</topic><topic>Chromatin - drug effects</topic><topic>Deoxyribonucleic acid</topic><topic>DNA</topic><topic>DNA fragmentation</topic><topic>DNA Fragmentation - drug effects</topic><topic>DNA integrity</topic><topic>Female</topic><topic>Fertility</topic><topic>Flow Cytometry</topic><topic>Fragmentation</topic><topic>Hogs</topic><topic>Integrity</topic><topic>Litter Size</topic><topic>Male</topic><topic>Morphology</topic><topic>Motility</topic><topic>Parameters</topic><topic>Parity</topic><topic>Pregnancy</topic><topic>Semen</topic><topic>semen analysis</topic><topic>Semen Analysis - veterinary</topic><topic>Semen Preservation - veterinary</topic><topic>Sperm</topic><topic>sperm chromatin structure assay</topic><topic>Spermatozoa - drug effects</topic><topic>Spermatozoa - physiology</topic><topic>Swine</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Myromslien, Frøydis D.</creatorcontrib><creatorcontrib>Tremoen, Nina H.</creatorcontrib><creatorcontrib>Andersen‐Ranberg, Ina</creatorcontrib><creatorcontrib>Fransplass, Ragnhild</creatorcontrib><creatorcontrib>Stenseth, Else‐Berit</creatorcontrib><creatorcontrib>Zeremichael, Teklu T.</creatorcontrib><creatorcontrib>van Son, Maren</creatorcontrib><creatorcontrib>Grindflek, Eli</creatorcontrib><creatorcontrib>Gaustad, Ann Helen</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Biotechnology Research Abstracts</collection><collection>Technology Research Database</collection><collection>Engineering Research Database</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Reproduction in domestic animals</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Myromslien, Frøydis D.</au><au>Tremoen, Nina H.</au><au>Andersen‐Ranberg, Ina</au><au>Fransplass, Ragnhild</au><au>Stenseth, Else‐Berit</au><au>Zeremichael, Teklu T.</au><au>van Son, Maren</au><au>Grindflek, Eli</au><au>Gaustad, Ann Helen</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Sperm DNA integrity in Landrace and Duroc boar semen and its relationship to litter size</atitle><jtitle>Reproduction in domestic animals</jtitle><addtitle>Reprod Domest Anim</addtitle><date>2019-02</date><risdate>2019</risdate><volume>54</volume><issue>2</issue><spage>160</spage><epage>166</epage><pages>160-166</pages><issn>0936-6768</issn><eissn>1439-0531</eissn><abstract><![CDATA[Contents
The sperm chromatin structure assay is a method for assessment of sperm DNA fragmentation, a parameter reported to be negatively related to field fertility in several mammal species. This method calculates a DNA fragmentation index (DFI) whose high values indicate abnormal chromatin structure. In this study, running from March 2010 until June 2017, the aim was to assess sperm DFI in stored liquid extended semen from two different pig breeds, Norwegian Landrace (NL; n = 693) and Norwegian Duroc (ND; n = 655), and to evaluate the influence on total number of piglets born (TNB). There was a significantly higher median DFI (p < 0.0001) in ejaculates from the 478 ND boars compared to the 452 NL boars. Data from 19,496 NL litters and 3,877 ND litters of the same boars were retrieved. For either breed, sow herd (p < 0.0001), parity (p < 0.05) and DFI (p < 0.05) showed significant effects on TNB. The DFI was negatively correlated to TNB in both breeds. The boars with the 5% lowest TNB had a least square means DFI of 3.05% and 2.24% in NL and ND, respectively, compared to 1.67% and 1.23% for the boars with the 5% highest TNB (p < 0.01). The DFI and the motility of the same semen samples were negatively correlated (p < 0.0001), and the high and low TNB groups showed significant differences in motility. However, this difference could not be used for practical prediction of TNB group (92.1% vs. 89.7%; p = 0.0038 and 92.3% vs. 89.5%; p = 0.018; NL and ND, respectively). In conclusion, our results indicate that sperm DNA integrity in semen with good motility and morphology may be an additional prediction parameter for fertility in pigs.]]></abstract><cop>Germany</cop><pub>Blackwell Publishing Ltd</pub><pmid>30168871</pmid><doi>10.1111/rda.13322</doi><tpages>7</tpages><orcidid>https://orcid.org/0000-0002-7873-734X</orcidid></addata></record> |
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| ispartof | Reproduction in domestic animals, 2019-02, Vol.54 (2), p.160-166 |
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| source | Wiley-Blackwell Read & Publish Collection |
| subjects | Acridine Orange Animals boar Breeding Chromatin Chromatin - chemistry Chromatin - drug effects Deoxyribonucleic acid DNA DNA fragmentation DNA Fragmentation - drug effects DNA integrity Female Fertility Flow Cytometry Fragmentation Hogs Integrity Litter Size Male Morphology Motility Parameters Parity Pregnancy Semen semen analysis Semen Analysis - veterinary Semen Preservation - veterinary Sperm sperm chromatin structure assay Spermatozoa - drug effects Spermatozoa - physiology Swine |
| title | Sperm DNA integrity in Landrace and Duroc boar semen and its relationship to litter size |
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