Efficient methodology for the cyclization of linear peptide libraries via intramolecular S-alkylation using MultipinTM solid phase peptide synthesis

Methodology is described here for the efficient parallel synthesis and cyclization of linear peptide libraries using intramolecular S-alkylation chemistry in combination with MultipinTM solid phase peptide synthesis (MultipinTM SPPS). The effective use of this methodology was demonstrated with the s...

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Bibliographic Details
Published in:Journal of peptide science 2006-08, Vol.12 (8), p.525-532
Main Authors: Roberts, Kade D, Lambert, John N, Ede, Nicholas J, Bray, Andrew M
Format: Article
Language:English
Online Access:Get full text
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Summary:Methodology is described here for the efficient parallel synthesis and cyclization of linear peptide libraries using intramolecular S-alkylation chemistry in combination with MultipinTM solid phase peptide synthesis (MultipinTM SPPS). The effective use of this methodology was demonstrated with the synthesis of a 72-member combinatorial library of cyclic thioether peptide derivatives of the conserved four-residue structural motif DD/EXK found in the active sites of the five crystallographically defined orthodox type II restriction endonucleases, EcoRV, EcoRI, PvuII, BamHI and BglI.
ISSN:1075-2617
DOI:10.1002/psc.761