A culture-independent approach for studying microbial diversity in aerobic granules

This study reports the use of ribosomal-based molecular techniques to study the microbial diversity in aerobic granules. Aerobic granules at different growth stages (young, mature and old) were obtained from a laboratory scale sequential aerobic sludge blanket (SASB) bioreactor fed with glucose as t...

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Bibliographic Details
Published in:Water science and technology 2003, Vol.47 (1), p.283-290
Main Authors: YI, S, TAY, J.-H, MASZENAN, A. M, TAY, S. T.-L
Format: Article
Language:English
Subjects:
Aerobic Digestion
Applied sciences
Bacteria, Aerobic - genetics
Biological and medical sciences
Biological treatment of waters
Bioreactors
Biotechnology
Composition
Deoxyribonucleic acid
DNA
DNA, Bacterial - analysis
Environment and pollution
Environmental Monitoring - methods
Exact sciences and technology
Fundamental and applied biological sciences. Psychology
Gene Library
General purification processes
Genetic Variation
Granular materials
Industrial applications and implications. Economical aspects
Microbial activity
Microorganisms
Nucleotide sequence
PCR
Pollution
Polymerase Chain Reaction
Polymorphism
Population Dynamics
Restriction fragment length polymorphism
Ribosomal DNA
RNA, Ribosomal, 16S
Sludge
Waste Disposal, Fluid
Wastewaters
Water treatment and pollution
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Summary:This study reports the use of ribosomal-based molecular techniques to study the microbial diversity in aerobic granules. Aerobic granules at different growth stages (young, mature and old) were obtained from a laboratory scale sequential aerobic sludge blanket (SASB) bioreactor fed with glucose as the main source of carbon and energy. Deoxyribonucleic acid (DNA) was extracted from the young, mature and old granules. The polymerase chain reaction (PCR) was used to amplify the Eubacterial 16S ribosomal DNA (rDNA) and three clone libraries were constructed, corresponding to each of the three growth stages. The microbial diversity in each clone library was assessed by amplified ribosomal DNA restriction analysis (ARDRA). The results reveal that there was considerable diversity in each clone library and there were variations in microbial diversity among the three different clone libraries. This suggests a shift in the composition of the microbial communities. Microorganisms associated with 5 restriction fragment length polymorphism (RFLP) types (A, B, C, D and E) appear to play an important role in the development of aerobic granules.
ISSN:0273-1223
1996-9732
DOI:10.2166/wst.2003.0068