Seasonal variations in sperm acrosome reaction, osmotic tolerance and serum testosterone concentrations in rams

The study was conducted to evaluate the seasonal dynamics in the sperm acrosome reaction (AR), osmotic tolerance and serum testosterone concentration in rams. Blood and semen samples were collected from six mature rams during the winter, spring, summer and autumn seasons. The AR in sperm samples was...

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Bibliographic Details
Published in:Animal reproduction science 2018-11, Vol.198, p.112-120
Main Authors: Ahmad, Ejaz, Naseer, Zahid, Uçan, Uğur, Serin, İlker, Ceylan, Ahmet, Aksoy, Melih
Format: Article
Language:English
Subjects:
Acrosome reaction
Acrosome Reaction - physiology
Animals
Male
Osmotic Pressure - physiology
Osmotic tolerance
Ram sperm
Seasons
Semen Analysis
Sheep - blood
Sheep - physiology
Sperm Motility
Spermatozoa - cytology
Spermatozoa - physiology
Testosterone
Testosterone - blood
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Summary:The study was conducted to evaluate the seasonal dynamics in the sperm acrosome reaction (AR), osmotic tolerance and serum testosterone concentration in rams. Blood and semen samples were collected from six mature rams during the winter, spring, summer and autumn seasons. The AR in sperm samples was induced by using lysophosphatidylcholine (LPC) and calcium ionophore (CI). The AR was monitored at various incubation times (15, 30, 60, 120 and 180 min) post-induction. To determine the osmotic tolerance through hypo-osmotic swelling test (HOST) the sperm were exposed to different osmotic conditions (50, 100, 290, 500 and 1000 mOsm/L). The serum testosterone concentration was assessed using a solid phase enzyme-linked immunosorbent assay (ELISA). The proportion of acrosome reacted sperm was greater (P 
ISSN:0378-4320
1873-2232
DOI:10.1016/j.anireprosci.2018.09.008