Evaluation of Paeonia emodi and its gold nanoparticles for cardioprotective and antihyperlipidemic potentials

• Published in: Journal of photochemistry and photobiology. B, Biology Biology, 2018-12, Vol.189, p.5-13
• Main Authors: Ibrar, Muhammad, Khan, Mir Azam, Abdullah, Imran, Muhammad
• Format: Article
• Language: English
• Subjects: Acetic acid; Alanine; Alanine transaminase; Animals; Anti-hyperlipidemia; Arteriosclerosis; Aspartate aminotransferase; Atherosclerosis; Atherosclerosis - drug therapy; Body weight; Cardio protective; Cardiotonic Agents - isolation & purification; Cardiovascular system; Column chromatography; Congestive heart failure; Constipation; Coronary artery disease; Creatine; Creatine kinase; Deoxyribonucleic acid; DNA; DNA damage; DNA fragmentation; DNA protective effect; Epilepsy; Ethyl acetate; Flowers & plants; Gold; Gold nanoparticles; Heart; Hypertension; Hypolipidemic Agents - isolation & purification; Isoproterenol; L-Lactate dehydrogenase; Lactate dehydrogenase; Lactic acid; Lipids; Metal Nanoparticles - therapeutic use; Mice; Myocardial infarction; Myocardial Infarction - drug therapy; Nanoparticles; Paeonia; Paeonia - chemistry; Paeonia emodi; Plant extracts; Plant Extracts - pharmacology; Respiratory diseases; Restoration; Serum levels; Weight reduction
• ISSN: 1011-1344; 1873-2682
• DOI: 10.1016/j.jphotobiol.2018.09.018

Paeonia emodi Wall. ex Royle is an important member of family Paeoniaceae and folklorically used for constipation, hysteria, respiratory diseases, epilepsy and cardiac diseases like hypertension, palpitations, congestive heart failure and atherosclerosis. In the present study, ethyl acetate fraction of P. emodi (Pe.EA) was subjected to column chromatography to obtain sub- fractions. These sub-fractions were screened for their cardioprotective activity in isoproterenol hydrochloride (ISO) induced myocardial infarction (MI) in mice. The most active fraction Pe. EA 40 was used for its gold nanoparticles synthesis (Pe.EA 40-AuNPs). Pe.EA 40 and Pe.EA 40-AuNPs were investigated for their cardioprotective, antihyperlipidemic, DNA fragmentation assay and histopathological study. Pe.EA 40 (80 mg/kg body weight) significantly reduced the serum levels of Alanine Aminotransferase (ALT), Aspartate Aminotransferase (AST), Lactate Dehydrogenase (LDH), Creatine Phosphokinase (CPK) to 66.07 ± 1.54, 77.08 ± 1.79, 84.86 ± 1.34 and 265.34 ± 4.34 IU/L respectively as compared to ISO treated group. Pe.EA 40-AuNPs (40 mg/kg) reduced the levels of ALT, AST, CPK and LDH to 60.74 ± 2.79, 75.47 ± 1.67, 80.48 ± 2.64 and 247.54. ± 5.57 IU/L respectively. A significant reduction was observed in lipid profile, protection in DNA damage and restoration of histopathological changes as compared to ISO treated group. Based on the results, it can be suggested that preparation of Pe.EA 40-AuNPs enhances the therapeutic potential of plant extract for the treatment of atherosclerosis and MI. [Display omitted] •Ethyl acetate fraction of Paeonia emodi was subjected to column chromatography.•The sub fractions obtained were investigated for cardioprotective effect.•Development of Gold nanoparticles (AuNPs) of the most active subfraction.•AuNPs significantly reduced lipid profile, ALT, AST, CPK, LDH and protected DNA damage.•AuNPs enhanced the therapeutic potential of extract for the treatment of atherosclerosis and MI.