Molecular analysis of biocompatibility of anodized titanium with deposited silver nanodendrites

• Published in: Materials Science & Engineering C 2018-12, Vol.93, p.437-444
• Main Authors: Kaczmarek, Mariusz, Jurczyk, Karolina, Purwin, Dominika, Koper, Jeremiasz K., Romaniuk, Aleksandra, Lipinska, Natalia, Jakubowicz, Jarosław, Jurczyk, Mieczyslawa U.
• Format: Article
• Language: English
• Subjects: Adhesion tests; Biocompatibility; Biomedical materials; Biotechnology; Cell Line, Tumor; Cell lines; Coated Materials, Biocompatible - chemistry; Coated Materials, Biocompatible - pharmacology; Dendrimers - chemistry; Dendrimers - pharmacology; Extracellular signal-regulated kinase; Fibroblasts; Fibroblasts - cytology; Fibroblasts - metabolism; Focal adhesion kinase; Focal Adhesion Kinase 1 - biosynthesis; Gene expression; Gene Expression Regulation - drug effects; Genes; Humans; Integrin alpha5 - biosynthesis; Integrin beta1 - biosynthesis; Integrins; Kinases; Materials science; Materials Testing; Metal Nanoparticles - chemistry; Mitogen-Activated Protein Kinase 3 - biosynthesis; Nanodendrites; Osteoblasts; Osteoblasts - cytology; Osteoblasts - metabolism; Real-time PCR; Silver; Silver - chemistry; Silver - pharmacology; Surgical implants; Titanium; Titanium - chemistry; Titanium - pharmacology
• ISSN: 0928-4931; 1873-0191
• DOI: 10.1016/j.msec.2018.08.001

Titanium (>99.6% purity) and its anodically oxidized modifications, with and without deposited silver nanodendrites regarding its biocompatibility were evaluated. In human gingival fibroblasts and osteoblast cell lines grown on tested samples, the level of expression of genes encoding αV (ITGAV) and β1 (ITGB1) integrin subunits also genes encoding focal adhesion (FAK) and extracellular-signal regulated (ERK) kinases was assessed. For this purpose, the qualitative and quantitative PCR technique was used. The expression of studied genes was dependent on the origin of cell lines and the type of evaluated material. The high expression of PBGD and ITGAV genes in fibroblasts grown on the surface of anodically modified titanium with deposited silver nanodendrites indicates potentially high biocompatibility of these samples for soft tissue cells. The high expression of the ITGB1 and ERK1 genes and the enhanced expression of the FAK gene in osteoblasts cells grown on the tested material was also observed. Summarizing, the nanocrystalline Ti modified with silver deposits showed higher biocompatibility in comparison with the conventional pure Ti samples. [Display omitted] •Modifications of materials influence on molecular biology of cells being in the contact with modified material•Changes affected the expression of genes regulating both of adhesion and signal transduction•Molecular analysis may serve as a method for assessment of biocompatibility the newly invented materials