Regulation of the inositol transporter Itr1p by hydrogen peroxide in Saccharomyces cerevisiae

Myo -inositol is a precursor of several membrane phospholipids and sphingolipids and plays a key role in gene regulation in Saccharomyces cerevisiae ( S. cerevisiae ). Here, we tested whether H 2 O 2 was affecting the levels of the inositol transporters and thus inositol uptake. In S. cerevisiae cel...

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Bibliographic Details
Published in:Archives of microbiology 2019, Vol.201 (1), p.123-134
Main Authors: Santos, Tomás, Marinho, H. Susana, Cyrne, Luisa
Format: Article
Language:English
Subjects:
Adaptation, Physiological
Baking yeast
Biochemistry
Biodegradation
Biological Transport - drug effects
Biological Transport - genetics
Biomedical and Life Sciences
Biotechnology
Cell Biology
Ecology
Gene expression
Gene regulation
Hydrogen peroxide
Hydrogen Peroxide - pharmacology
Inositol
Inositol - metabolism
Internalization
Intracellular levels
Life Sciences
Membrane Transport Proteins - drug effects
Membrane Transport Proteins - genetics
Membranes
Microbial Ecology
Microbiology
Original Paper
Phenotypes
Phospholipids
Saccharomyces cerevisiae
Saccharomyces cerevisiae - genetics
Saccharomyces cerevisiae - metabolism
Saccharomyces cerevisiae Proteins - drug effects
Saccharomyces cerevisiae Proteins - genetics
Sphingolipids
Yeast
Yeasts
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Summary:Myo -inositol is a precursor of several membrane phospholipids and sphingolipids and plays a key role in gene regulation in Saccharomyces cerevisiae ( S. cerevisiae ). Here, we tested whether H 2 O 2 was affecting the levels of the inositol transporters and thus inositol uptake. In S. cerevisiae cells adapted to H 2 O 2 Itr1–GFPp accumulated in the plasma membrane until 20 min, concomitantly with an inhibition of its internalization. Exposure to H 2 O 2 did not alter Itr2–GFPp cellular levels and induced only an 8% decrease at 10 min in the plasma membrane. Therefore, decreased inositol intracellular levels are not caused by decreased levels of inositol transporters in the plasma membrane. However, results show that H 2 O 2 adaptation affects Itr1p turnover and, consequently, H 2 O 2 -adapted yeast cells display an inositol transporter phenotype comparable to cells grown in the absence of inositol in growth medium, i.e. accumulation in the plasma membrane and decreased degradation.
ISSN:0302-8933
1432-072X
DOI:10.1007/s00203-018-1584-1