Deadenylation by the CCR4‐NOT complex contributes to the turnover of hairy‐related mRNAs in the zebrafish segmentation clock

In the zebrafish segmentation clock, hairy/enhancer of split‐related genes her1, her7, and hes6 encodes components of core oscillators. Since the expression of cyclic genes proceeds rapidly in the presomitic mesoderm (PSM), these hairy‐related mRNAs are subject to strict post‐transcriptional regulat...

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Bibliographic Details
Published in:FEBS letters 2018-10, Vol.592 (20), p.3388-3398
Main Authors: Fujino, Yuuri, Yamada, Kazuya, Sugaya, Chihiro, Ooka, Yuko, Ovara, Hiroki, Ban, Hiroyuki, Akama, Kagari, Otosaka, Shiori, Kinoshita, Hirofumi, Yamasu, Kyo, Mishima, Yuichiro, Kawamura, Akinori
Format: Article
Language:English
Subjects:
3′UTR
Animals
Animals, Genetically Modified
Basic Helix-Loop-Helix Transcription Factors - genetics
Basic Helix-Loop-Helix Transcription Factors - metabolism
Biological Clocks
Body Patterning - genetics
deadenylation
decapping
Exoribonucleases - genetics
Exoribonucleases - metabolism
Gene Expression Regulation, Developmental
Mesoderm - embryology
Mesoderm - metabolism
mRNA decay
RNA Processing, Post-Transcriptional
RNA, Messenger - genetics
RNA, Messenger - metabolism
somite segmentation
Somites - embryology
Somites - metabolism
Transcription Factors - genetics
Transcription Factors - metabolism
zebrafish
Zebrafish - embryology
Zebrafish - genetics
Zebrafish - metabolism
Zebrafish Proteins - genetics
Zebrafish Proteins - metabolism
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Summary:In the zebrafish segmentation clock, hairy/enhancer of split‐related genes her1, her7, and hes6 encodes components of core oscillators. Since the expression of cyclic genes proceeds rapidly in the presomitic mesoderm (PSM), these hairy‐related mRNAs are subject to strict post‐transcriptional regulation. In this study, we demonstrate that inhibition of the CCR4‐NOT deadenylase complex lengthens poly(A) tails of hairy‐related mRNAs and increases the amount of these mRNAs, which is accompanied by defective somite segmentation. In transgenic embryos, we show that EGFP mRNAs with 3′UTRs of hairy‐related genes exhibit turnover similar to endogenous mRNAs. Our results suggest that turnover rates of her1, her7, and hes6 mRNAs are differently regulated by the CCR4‐NOT deadenylase complex possibly through their 3′UTRs in the zebrafish PSM.
ISSN:0014-5793
1873-3468
DOI:10.1002/1873-3468.13261