Clinical significance of aberrant cyclin-dependent kinase-like 2 methylation in hepatocellular carcinoma

Aberrant DNA methylation of cyclin-dependent kinase-like 2 (CDKL2) had been observed in several types of tumors. Herein, the present study was aimed to explore the epigenetic and expression status of CDKL2 and evaluate the diagnostic potential of CDKL2 methylation in hepatocellular carcinoma (HCC)....

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Bibliographic Details
Published in:Gene 2019-01, Vol.683, p.35-40
Main Authors: Zhou, Ye, Qiu, Xue-ping, Li, Zu-hua, Zhang, Shuai, Rong, Yuan, Yang, Guo-hua, Fang-Zheng
Format: Article
Language:English
Subjects:
CDKL2
DNA methylation
HCC
mRNA expression
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Summary:Aberrant DNA methylation of cyclin-dependent kinase-like 2 (CDKL2) had been observed in several types of tumors. Herein, the present study was aimed to explore the epigenetic and expression status of CDKL2 and evaluate the diagnostic potential of CDKL2 methylation in hepatocellular carcinoma (HCC). The methylation status of CDKL2 was detected by methylation-sensitive restriction enzyme based quantitative PCR (MSRE-qPCR) and bisulfite genomic sequencing (BGS). The mRNA expression of CDKL2 was measured using real-time quantitative PCR (qPCR). The correlations between the methylation of CDKL2 and mRNA expression, clinicopathological features were evaluated. Compared with normal liver tissues, the methylation levels of CDKL2 were significantly increased in the HCC tissues and cell lines (All p 
ISSN:0378-1119
1879-0038
DOI:10.1016/j.gene.2018.10.009