Alterations in the diversity and composition of mice gut microbiota by lytic or temperate gut phage treatment

Phages, the most abundant species in the mammalian gut, have numerous advantages as biocontrol agent over antibiotics. In this study, mice were orally treated with the lytic gut phage PA13076 (group B), the temperate phage BP96115 (group C), no phage (group A), or streptomycin (group D) over 31 days...

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Bibliographic Details
Published in:Applied microbiology and biotechnology 2018-12, Vol.102 (23), p.10219-10230
Main Authors: Bao, Hong-duo, Pang, Mao-da, Olaniran, Ademola, Zhang, Xu-hui, Zhang, Hui, Zhou, Yan, Sun, Li-chang, Schmidt, Stefan, Wang, Ran
Format: Article
Language:English
Subjects:
Abundance
Animals
Antibiotics
Applied Microbial and Cell Physiology
Bacteria
Bacteriolysis
Bacteriophages - physiology
Bacteroidetes - drug effects
Bacteroidetes - virology
Bifidobacterium - drug effects
Bifidobacterium - virology
Biological control
Biomedical and Life Sciences
Biotechnology
Composition
Escherichia
Escherichia - drug effects
Escherichia - virology
Fecal microflora
Feces - microbiology
Female
Firmicutes - drug effects
Firmicutes - virology
Gastrointestinal Microbiome - drug effects
Gene sequencing
High-Throughput Nucleotide Sequencing
Intestinal microflora
Klebsiella
Klebsiella - drug effects
Klebsiella - virology
Lactobacillus - drug effects
Lactobacillus - virology
Life Sciences
Mice
Mice, Inbred C57BL
Microbial Genetics and Genomics
Microbiology
Microbiota
Next-generation sequencing
Phages
RNA, Ribosomal, 16S - genetics
rRNA 16S
Sequence Analysis, DNA
Shigella
Shigella - drug effects
Shigella - virology
Streptomycin
Streptomycin - pharmacology
Variable region
Virus-like particles
Viruses
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Description
Summary:Phages, the most abundant species in the mammalian gut, have numerous advantages as biocontrol agent over antibiotics. In this study, mice were orally treated with the lytic gut phage PA13076 (group B), the temperate phage BP96115 (group C), no phage (group A), or streptomycin (group D) over 31 days. At the end of the experiment, fecal microbiota diversity and composition was determined and compared using high-throughput sequencing of the V3–V4 hyper-variable region of the 16S rRNA gene and virus-like particles (VLPs) were quantified in feces. There was high diversity and richness of microbiota in the lytic and temperate gut phage-treated mice, with the lytic gut phage causing an increased alpha diversity based on the Chao1 index ( p  
ISSN:0175-7598
1432-0614
DOI:10.1007/s00253-018-9378-6