Production and characterization of an amphidiploid line between Brassica rapa and a wild relative Diplotaxis tenuifolia

• Published in: Plant breeding 2009-10, Vol.128 (5), p.536-537
• Main Authors: Jeong, B.H, Saga, T, Okayasu, K, Hattori, G, Kaneko, Y, Bang, S.W
• Format: Article
• Language: English
• Subjects: Agronomy. Soil science and plant productions; amphidiploid line; Biological and medical sciences; Brassica rapa; Cytogenetics; Diplotaxis; Diplotaxis tenuifolia; embryo rescue; Fundamental and applied biological sciences. Psychology; Generalities. Genetics. Plant material; Genetics and breeding of economic plants; intergeneric hybrid; Interspecific and intergeneric hybridization, introgressions; Plant breeding: fundamental aspects and methodology
• ISSN: 0179-9541; 1439-0523
• DOI: 10.1111/j.1439-0523.2008.01517.x

Intergeneric hybridization was performed between Brassica rapa and Diplotaxis tenuifolia following embryo rescue. Twenty-three F₁ hybrid plants were developed from the cross B. rapa x D. tenuifolia and confirmed to be amphihaploids with 21 chromosomes in mitosis. Chromosome doubling of F₁ hybrids by colchicine treatment resulted into five amphidiploid plants which exhibited (20-21)II + (0-2)I at metaphase I (MI) of pollen mother cells. Sib-crossing and/or open-pollination among amphidiploid plants for more than four generations resulted in the development of an ADt-06 line with reproductive systems capable of maintaining an amphidiploid line. The ADt-06 line was intermediate in some morphological traits between two parental species, and was characterized by a slightly pungent taste as a physiological trait. Analyses for genomic DNA confirmed that this line was a hybrid between two species. This new amphidiploid ADt-06 line could be a useful genetic resource for the breeding of new leafy salad vegetables.