Low-level arsenite activates the transcription of genes involved in adipose differentiation

In this study we analyzed gene expression in 3T3-F442A pre-adipocyte cells that differentiate in the presence of micro-molar arsenate concentration. Two concentrations of arsenite (As2O3, 0.25 micromol/L and 0.5 micromol/L) were applied for three days with and without insulin (170 nmol/L) and gene e...

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Bibliographic Details
Published in:Cell biology and toxicology 2004-11, Vol.20 (6), p.375-385
Main Authors: Salazard, B, Bellon, L, Jean, S, Maraninchi, M, El-Yazidi, C, Orsière, T, Margotat, A, Botta, A, Bergé-Lefranc, J-L
Format: Article
Language:English
Subjects:
3T3 Cells
3T3-L1 Cells
Adipocytes - cytology
Adipocytes - drug effects
Animals
Arsenites - pharmacology
CCAAT-Enhancer-Binding Protein-alpha - genetics
Cell Differentiation - genetics
Cell Line
Cell Proliferation - drug effects
Cell Survival - drug effects
Dose-Response Relationship, Drug
Gene expression
Gene Expression - drug effects
Gene Expression - genetics
Heme Oxygenase (Decyclizing) - genetics
Heme Oxygenase-1
Hypoxia
Hypoxia-Inducible Factor 1, alpha Subunit
Kruppel-Like Transcription Factors
Membrane Proteins
Mice
Oxidative stress
PPAR gamma - genetics
Proteins
Reverse Transcriptase Polymerase Chain Reaction
Teratogens - pharmacology
Trans-Activators - genetics
Transcription Factors - genetics
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Summary:In this study we analyzed gene expression in 3T3-F442A pre-adipocyte cells that differentiate in the presence of micro-molar arsenate concentration. Two concentrations of arsenite (As2O3, 0.25 micromol/L and 0.5 micromol/L) were applied for three days with and without insulin (170 nmol/L) and gene expressions were evaluated by quantitative RT-PCR. The genes included genes of oxidative-stress responses: heme-oxygenase-1 (HO1) and the hypoxia inducible factor 1a (HIF1alpha), genes of cell-cycle: c-jun and Kruppel like factor 5 (KLF5), and genes that play important roles in adipose determination: a peroxisome proliferator-activated receptor (PPARgamma) and a CCAAT/ enhancer binding protein (C/EBPalpha). Arsenite induced the expression of HO1, HIF1alpha, KLF5, PPARgamma and C/EBPalpha. These results suggest that under condition of oxidative stress arsenite induces genes that are required for adipose differentiation.
ISSN:0742-2091
1573-6822
DOI:10.1007/s10565-004-1471-1