Early adipose deposits in sheep: comparative analysis of the perirenal fat transcriptome of Assaf and Churra suckling lambs

• Published in: Animal genetics 2018-12, Vol.49 (6), p.605-617
• Main Authors: Suárez‐Vega, A., Arranz, J. J., Pérez, V., Fuente, L. F., Mateo, J., Gutiérrez‐Gil, B.
• Format: Article
• Language: English
• Subjects: Adipocytes; Adipose tissue; Adipose Tissue, Brown; Adipose Tissue, White; Animals; Biological activity; Biomarkers; Body fat; Breeding; Burning; carcass traits; Carcasses; Cholesterol; Comparative analysis; Deposits; Enrichment; Fragments; Gene expression; Genes; Intra-Abdominal Fat; Kidney; Lipid Metabolism; Male; Meat; Oxidation; Ribonucleic acid; RNA; RNA‐seq; Sequence Analysis, RNA; Sheep; Sheep - genetics; Suckling behavior; Transcriptome; Uncoupling protein 1
• ISSN: 0268-9146; 1365-2052
• DOI: 10.1111/age.12725

Summary Adipose deposits influence the quality of ruminant carcasses, and in suckling lambs, internal types of adipose deposits represent a notable proportion of total fat. The aim of this study was to perform a comparative analysis of the perirenal fat transcriptomes of suckling lambs from two breeds with different growth and carcass characteristics. The perirenal fat tissue from 14 suckling lambs (Assaf, n = 8; Churra, n = 6) was used for the RNA‐seq analysis. The functional enrichment analysis of the 670 highly expressed genes (>150 fragments per kilobase of exon per million fragments mapped) in the perirenal fat transcriptome of both breeds revealed that the majority of these genes were involved in energy processes. The expression of the UCP1 gene, a classical biomarker of brown fat, and the presence of multilocular adipocytes in the two breeds supported the presence of brown fat at the transition stage towards white fat tissue. The differential expression analysis performed identified 373 differentially expressed genes (DEGs) between the two compared breeds. Brown/white fat gene biomarkers were not included in the list of DEGs. In Assaf lambs, DEGs were enriched in Gene Ontology (GO) biological processes related to fatty‐acid oxidation, whereas in Churra lambs, the majority of the significantly enriched GO terms were related to cholesterol synthesis, which suggests that upregulated DEGs in Assaf lambs are implicated in fat burning, whereas the Churra upregulated DEGs are linked to fat accumulation. These results can help to increase knowledge of the genes controlling early fat deposition in ruminants and shed light on fundamental aspects of adipose tissue growth.