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Early adipose deposits in sheep: comparative analysis of the perirenal fat transcriptome of Assaf and Churra suckling lambs

Summary Adipose deposits influence the quality of ruminant carcasses, and in suckling lambs, internal types of adipose deposits represent a notable proportion of total fat. The aim of this study was to perform a comparative analysis of the perirenal fat transcriptomes of suckling lambs from two bree...

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Published in:Animal genetics 2018-12, Vol.49 (6), p.605-617
Main Authors: Suárez‐Vega, A., Arranz, J. J., Pérez, V., Fuente, L. F., Mateo, J., Gutiérrez‐Gil, B.
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container_title Animal genetics
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description Summary Adipose deposits influence the quality of ruminant carcasses, and in suckling lambs, internal types of adipose deposits represent a notable proportion of total fat. The aim of this study was to perform a comparative analysis of the perirenal fat transcriptomes of suckling lambs from two breeds with different growth and carcass characteristics. The perirenal fat tissue from 14 suckling lambs (Assaf, n = 8; Churra, n = 6) was used for the RNA‐seq analysis. The functional enrichment analysis of the 670 highly expressed genes (>150 fragments per kilobase of exon per million fragments mapped) in the perirenal fat transcriptome of both breeds revealed that the majority of these genes were involved in energy processes. The expression of the UCP1 gene, a classical biomarker of brown fat, and the presence of multilocular adipocytes in the two breeds supported the presence of brown fat at the transition stage towards white fat tissue. The differential expression analysis performed identified 373 differentially expressed genes (DEGs) between the two compared breeds. Brown/white fat gene biomarkers were not included in the list of DEGs. In Assaf lambs, DEGs were enriched in Gene Ontology (GO) biological processes related to fatty‐acid oxidation, whereas in Churra lambs, the majority of the significantly enriched GO terms were related to cholesterol synthesis, which suggests that upregulated DEGs in Assaf lambs are implicated in fat burning, whereas the Churra upregulated DEGs are linked to fat accumulation. These results can help to increase knowledge of the genes controlling early fat deposition in ruminants and shed light on fundamental aspects of adipose tissue growth.
doi_str_mv 10.1111/age.12725
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In Assaf lambs, DEGs were enriched in Gene Ontology (GO) biological processes related to fatty‐acid oxidation, whereas in Churra lambs, the majority of the significantly enriched GO terms were related to cholesterol synthesis, which suggests that upregulated DEGs in Assaf lambs are implicated in fat burning, whereas the Churra upregulated DEGs are linked to fat accumulation. These results can help to increase knowledge of the genes controlling early fat deposition in ruminants and shed light on fundamental aspects of adipose tissue growth.</abstract><cop>England</cop><pub>Wiley Subscription Services, Inc</pub><pmid>30311245</pmid><doi>10.1111/age.12725</doi><tpages>13</tpages><orcidid>https://orcid.org/0000-0001-9058-131X</orcidid><orcidid>https://orcid.org/0000-0001-7990-5723</orcidid></addata></record>
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ispartof Animal genetics, 2018-12, Vol.49 (6), p.605-617
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recordid cdi_proquest_miscellaneous_2119916799
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subjects Adipocytes
Adipose tissue
Adipose Tissue, Brown
Adipose Tissue, White
Animals
Biological activity
Biomarkers
Body fat
Breeding
Burning
carcass traits
Carcasses
Cholesterol
Comparative analysis
Deposits
Enrichment
Fragments
Gene expression
Genes
Intra-Abdominal Fat
Kidney
Lipid Metabolism
Male
Meat
Oxidation
Ribonucleic acid
RNA
RNA‐seq
Sequence Analysis, RNA
Sheep
Sheep - genetics
Suckling behavior
Transcriptome
Uncoupling protein 1
title Early adipose deposits in sheep: comparative analysis of the perirenal fat transcriptome of Assaf and Churra suckling lambs
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