pH: the silent variable significantly impacting meiotic spindle assembly in mouse oocytes

Temperature fluctuation negatively impacts the assembly and function of the meiotic spindle, but does pH have a similar effect? Polarized light microscopy was used to study the spindle in living mouse oocytes under different pH conditions. Female mice (n = 53) were superovulated, and oocytes collect...

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Bibliographic Details
Published in:Reproductive biomedicine online 2018-09, Vol.37 (3), p.279-290
Main Authors: Swearman, Harriet, Koustas, George, Knight, Emily, Liperis, Georgios, Grupen, Christopher, Sjoblom, Cecilia
Format: Article
Language:English
Subjects:
Animals
Female
Hydrogen-Ion Concentration
Meiosis - physiology
meiotic spindle
Mice
oocyte
Oocytes - physiology
polarized light microscopy
retardance
Spindle Apparatus - physiology
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Summary:Temperature fluctuation negatively impacts the assembly and function of the meiotic spindle, but does pH have a similar effect? Polarized light microscopy was used to study the spindle in living mouse oocytes under different pH conditions. Female mice (n = 53) were superovulated, and oocytes collected, denuded and allocated to treatment groups. All experiments were performed at 37°C, and standard bicarbonate-buffered medium was used either pre-equilibrated in 6% CO2 or unequilibrated (in ambient CO2). Mean oocyte spindle retardance was measured over time in response to changing pH. Spindles were also assessed to understand whether this effect was reversible, by using a fixed pH in a zwitterionic buffer. The data show the spindle is impacted by pH fluctuation, with mean retardance significantly higher at pH 7.4–7.5 than at the point of media equilibration (P 
ISSN:1472-6483
1472-6491
DOI:10.1016/j.rbmo.2018.06.022