Microbial degradation of a single branched isomer of nonylphenol by Sphingomonas TTNP3

The endocrine disrupting chemical nonylphenol (NP) is a technical product which consists of a complex mixture of nonylphenols with different alkyl side-chain isomers. Since the bio-degradation of each NP isomer may lead to its own range of metabolites, the isolation and identification of transformat...

Full description

Saved in:
Bibliographic Details
Published in:Water science and technology 2004-01, Vol.50 (5), p.189-194
Main Authors: Corvini, P F X, Vinken, R, Hommes, G, Mundt, M, Hollender, J, Meesters, R, Schröder, H Fr, Schmidt, B
Format: Article
Language:English
Subjects:
Biodegradation
Biodegradation, Environmental
Biomass
Carbon Dioxide - chemistry
Carbon Dioxide - metabolism
Carbon Radioisotopes
Cells, Cultured
Chains
Culture techniques
Degradation
Endocrine disruptors
Fermentation
Gas Chromatography-Mass Spectrometry
Isomerism
Isomers
Metabolites
Microbial degradation
Microorganisms
Mineralization
Phenols
Phenols - chemical synthesis
Phenols - metabolism
Radioactivity
Sewage - microbiology
Soil Microbiology
Spectrometry, Mass, Electrospray Ionization
Sphingomonas
Sphingomonas - metabolism
Uranium
Citations: Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:The endocrine disrupting chemical nonylphenol (NP) is a technical product which consists of a complex mixture of nonylphenols with different alkyl side-chain isomers. Since the bio-degradation of each NP isomer may lead to its own range of metabolites, the isolation and identification of transformation products is very difficult. In order to overcome this difficulty, the nonylphenol isomer 4(3',5'-dimethyl-3'-heptyl)-phenol (p353NP) was synthesized, and its degradation by an axenic culture of Sphingomonas TTNP3 was investigated with [ring-U-14C]-labelled and non-labelled p353NP including a time-course study. Radioactive mass balancing resulted in different polar soluble fractions, in insoluble radioactivity associated with biomass, and volatile radioactivity in the form of the mineralization product 14CO2. In the extracellular media, the presence of nonanol corresponding to the nonyl chain of the NP isomer was confirmed and its concentration was determined during the course of fermentation. No other radioactive compounds were detected beside the parent isomer. Radioactive metabolites were only found in the intracellular fraction of S. TTNP3.
ISSN:0273-1223
1996-9732
DOI:10.2166/wst.2004.0327