Deep line-temporal focusing with high axial resolution and a large field-of-view using intracavity control and incoherent pulse shaping

Line-temporal focusing has been recognized as an elegant strategy that provides two-photon microscopy with an effective means for fast imaging through parallelization, together with an improved resilience to scattering for deep imaging. However, the axial resolution remains sub-optimal, except when...

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Bibliographic Details
Published in:Optics letters 2018-10, Vol.43 (20), p.4919-4922
Main Authors: Lou, Kai, Wang, Bo, Jee, Ah-Young, Granick, Steve, Amblard, François
Format: Article
Language:English
Subjects:
Field of view
Imaging
Immunity
Microscopy
Scattering
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Summary:Line-temporal focusing has been recognized as an elegant strategy that provides two-photon microscopy with an effective means for fast imaging through parallelization, together with an improved resilience to scattering for deep imaging. However, the axial resolution remains sub-optimal, except when using high NA objectives and a small field-of-view. With the introduction of an intracavity control of the spectral width of the femtosecond laser to adaptively fill the back aperture of the objective lens, line-temporal focusing two-photon microscopy is demonstrated to reach near-diffraction-limited axial resolution with a large back-aperture objective lens, and improved immunity to sample scattering. In addition, a new incoherent flattop beam shaping method is proposed which provides a uniform contrast with little degradation of the axial resolution along the focus line, even deep in the sample. This is demonstrated in large volumetric imaging of mouse lung samples.
ISSN:0146-9592
1539-4794
DOI:10.1364/OL.43.004919