ZrMOF nanoparticles as quenchers to conjugate DNA aptamers for target-induced bioimaging and photodynamic therapy

Porphyrinic metal-organic framework (MOF) nanoparticles for photodynamic therapy solve the photosensitizer problems of poor solubility, self-quenching and aggregation. However, their low selectivity towards malignant tissues is an obstacle for bioimaging and a bottle-neck to cellular uptake for high...

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Bibliographic Details
Published in:Chemical science (Cambridge) 2018, Vol.9 (38), p.7505-7509
Main Authors: Liu, Yuan, Hou, Weijia, Xia, Lian, Cui, Cheng, Wan, Shuo, Jiang, Ying, Yang, Yu, Wu, Qiong, Qiu, Liping, Tan, Weihong
Format: Article
Language:English
Subjects:
Binding
Conjugates
Conjugation
Deoxyribonucleic acid
DNA
Medical imaging
Metal-organic frameworks
Nanomaterials
Nanoparticles
Photodynamic therapy
Quenching
Solid phases
Target recognition
Zirconium
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Summary:Porphyrinic metal-organic framework (MOF) nanoparticles for photodynamic therapy solve the photosensitizer problems of poor solubility, self-quenching and aggregation. However, their low selectivity towards malignant tissues is an obstacle for bioimaging and a bottle-neck to cellular uptake for highly efficient photodynamic therapy of cancer. Here, ZrMOF nanoparticles as quenchers to conjugate DNA aptamers were developed for target-induced bioimaging and photodynamic therapy. A phosphate-terminal aptamer prepared by solid-phase DNA synthesis was anchored on the surface of ZrMOF nanoparticles through strong coordination between phosphate and zirconium. Based on π-π stacking-induced quenching of TAMRA by ZrMOF nanoparticles, target-induced imaging is achieved due to the structural change of the aptamer upon binding with the target. Aptamer-conjugated ZrMOF nanoparticles with target binding ability significantly enhanced the photodynamic therapy effect. Furthermore, phosphate-terminal aptamer conjugation method can be generalized to other types of MOF nanomaterials, such as UiO-66 and HfMOF nanoparticles, which can be potentially used in biochemistry.
ISSN:2041-6520
2041-6539
DOI:10.1039/c8sc02210k