LINC01433 promotes hepatocellular carcinoma progression via modulating the miR‐1301/STAT3 axis

• Published in: Journal of cellular physiology 2019-05, Vol.234 (5), p.6116-6124
• Main Authors: Huang, Haijin, Bu, Yan‐Zhi, Zhang, Xiao‐Yu, Liu, Juan, Zhu, Li‐Yao, Fang, Yong
• Format: Article
• Language: English
• Subjects: Animals; Apoptosis; Bioinformatics; Biomarkers; Carcinoma, Hepatocellular - genetics; Carcinoma, Hepatocellular - pathology; Cell growth; Cell proliferation; Cell Proliferation - genetics; Colonies; Correlation analysis; Disease Progression; Gene Expression Regulation, Neoplastic - genetics; Hep G2 Cells; Hepatocellular carcinoma; hepatocellular carcinoma (HCC); Humans; LINC01433; Liver cancer; Liver Neoplasms - genetics; Liver Neoplasms - pathology; Lung cancer; Mice; Mice, Inbred BALB C; MicroRNAs - genetics; MicroRNAs - metabolism; miR‐1301; Ribonucleic acid; RNA; RNA, Long Noncoding - genetics; Signal Transduction - physiology; STAT3; Stat3 protein; STAT3 Transcription Factor - genetics; STAT3 Transcription Factor - metabolism
• ISSN: 0021-9541; 1097-4652
• DOI: 10.1002/jcp.27366

Long noncoding RNAs (lncRNAs) have been demonstrated to play significant roles in hepatocellular carcinoma (HCC) tumor progression. LINC01433 has been implicated in the progression of lung cancer. However, its biological role in HCC remains poorly understood. In our current study, we focused on the detailed mechanism of LINC01433 in HCC development. First, it was exhibited that LINC01433 was remarkably elevated in HCC cells, which indicated that LINC01433 was involved in HCC. Then, knockdown of LINC01433 was able to restrain HCC cell proliferation and cell colony formation and greatly induced cell apoptosis. On the contrary, overexpression of LINC01433 promoted HCC cell proliferation, increased cell colony formation, and enhanced cell invasion capacity. Subsequently, we found that miR‐1301 was remarkably decreased in HCC cells, and it can serve as a target of LINC01433 according to bioinformatics analysis. In addition, the binding correlation between them was validated by performing RNA pull‐down experiments and RIP assay. Moreover, STAT3 was predicted and validated as a target of miR‐1301, and it was shown that miR‐1301 mimics significantly suppressed STAT3 in HCC cells. Finally, in vivo models were established, and the results demonstrated that silencing of LINC01433 could repress HCC development through modulating miR‐1301 and STAT3. Taken together, these results indicated in our study that LINC01433 participated in HCC progression through modulating the miR‐1301/STAT3 axis and it might act as a novel biomarker in HCC diagnosis and treatment. In our study, we found that silencing of LINC01433 repressed hepatocellular carcinoma (HCC) progression by sponging miR‐1301. In addition, STAT3 could act as a direct target of miR‐1301, and miR‐1301 mimics inhibited STAT3 expression significantly in HCC cells. Our data suggested that the LINC01433/miR‐1301/STAT3 axis was involved in HCC progression and LINC01433 might serve as a biomarker for HCC.