Hypermethylation of ITGBL1 is associated with poor prognosis in acute myeloid leukemia

The current study was aimed to investigate integrin beta‐like 1 (ITGBL1) methylation pattern and its clinical relevance in patients with acute myeloid leukemia (AML). Real‐time methylation‐specific polymerase chain reaction (PCR; RQ‐MSP) and bisulfite sequencing PCR (BSP) were performed to detect th...

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Bibliographic Details
Published in:Journal of cellular physiology 2019-06, Vol.234 (6), p.9438-9446
Main Authors: Lian, Xin‐Yue, Ma, Ji‐Chun, Zhou, Jing‐Dong, Zhang, Ting‐Juan, Wu, De‐Hong, Deng, Zhao‐Qun, Zhang, Zhi‐Hui, Li, Xi‐Xi, He, Pin‐Fang, Yan, Yang, Lin, Jiang, Qian, Jun
Format: Article
Language:English
Subjects:
Acute myeloid leukemia
Adult
Aged
Aged, 80 and over
Azacitidine - pharmacology
Biomarkers
Bisulfite
Bone marrow
Case-Control Studies
Demethylation
DNA methylation
DNA Methylation - drug effects
DNA Methylation - genetics
expression
Female
Follow-Up Studies
Gene Expression Regulation, Leukemic - drug effects
Humans
Induction therapy
Integrin beta1 - genetics
Integrin beta1 - metabolism
ITGBL1
K562 Cells
Leukemia
Leukemia, Myeloid, Acute - genetics
Male
Medical prognosis
Methylation
Middle Aged
Multivariate Analysis
Myeloid leukemia
Patients
Polymerase chain reaction
Prognosis
Promoter Regions, Genetic - genetics
Promyeloid leukemia
Remission
Survival
Survival Analysis
Transcription
Young Adult
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Summary:The current study was aimed to investigate integrin beta‐like 1 (ITGBL1) methylation pattern and its clinical relevance in patients with acute myeloid leukemia (AML). Real‐time methylation‐specific polymerase chain reaction (PCR; RQ‐MSP) and bisulfite sequencing PCR (BSP) were performed to detect the methylation of ITGBL1 promoter. Real‐time quantitative PCR (RT‐qPCR) was performed to analyze ITGBL1 transcript level. The results showed that ITGBL1 methylation level in 131 patients with AML was significantly higher than 29 controls (p 
ISSN:0021-9541
1097-4652
DOI:10.1002/jcp.27629