Fine-scale differences between Accumulibacter-like bacteria in enhanced biological phosphorus removal activated sludge

A lab-scale sequencing batch reactor (SBR) and six full-scale wastewater treatment plants (WWTPs) performing enhanced biological phosphorus removal (EBPR) were surveyed. The abundance of Accumulibacter-related organisms in the full-scale plants was investigated using fluorescent in situ hybridizatio...

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Bibliographic Details
Published in:Water science and technology 2006-01, Vol.54 (1), p.111-117
Main Authors: He, S, Gu, A Z, McMahon, K D
Format: Article
Language:English
Subjects:
Abundance
Activated Sludge
Bacteria
Batch reactors
Clustering
Environmental Restoration and Remediation
Fluorescence
Organisms
Phosphorus
Phosphorus - isolation & purification
Phosphorus removal
Phylogeny
Proteobacteria - genetics
Proteobacteria - metabolism
RNA, Bacterial - genetics
RNA, Ribosomal, 16S - genetics
Sewage - microbiology
Wastewater treatment
Wastewater treatment plants
Water treatment plants
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Summary:A lab-scale sequencing batch reactor (SBR) and six full-scale wastewater treatment plants (WWTPs) performing enhanced biological phosphorus removal (EBPR) were surveyed. The abundance of Accumulibacter-related organisms in the full-scale plants was investigated using fluorescent in situ hybridization. Accumulibacter-related organisms were present in all of the full-scale EBPR plants, at levels ranging from 9% to 24% of total cells. The high percentage of Accumulibacter-related organisms seemed to be associated with configurations which minimize the nitrate recycling to the anaerobic zone and low influent BOD:TP ratios. PCR-based clone libraries were constructed from the community 16S rRNA gene plus the internally transcribed spacer region amplified from the SBR and five of the full-scale WWTPs. Comparative sequence analysis was carried out using Accumulibacter-related clones, providing higher phylogenetic resolution and revealing finer-scale clustering of the sequences retrieved from the SBR and full-scale EBPR
ISSN:0273-1223
1996-9732
DOI:10.2166/wst.2006.378