Probiotic Clostridium butyricum Improves the Growth Performance, Immune Function, and Gut Microbiota of Weaning Rex Rabbits

• Published in: Probiotics and antimicrobial proteins 2019-12, Vol.11 (4), p.1278-1292
• Main Authors: Liu, Lei, Zeng, Dong, Yang, Mingyue, Wen, Bin, Lai, Jing, Zhou, Yi, Sun, Hao, Xiong, Lvcheng, Wang, Jie, Lin, Yicen, Pan, Kangcheng, Jing, Bo, Wang, Ping, Ni, Xueqin
• Format: Article
• Language: English
• Subjects: Antioxidants; Applied Microbiology; Chemistry; Chemistry and Materials Science; Chemistry/Food Science; Clostridium butyricum; Colon; Digestive system; Enzymatic activity; Gastrointestinal tract; Immune response; Inflammation; Interleukin 6; Intestinal microflora; Intestine; Microbiology; Microbiota; mRNA; Mucosa; MyD88 protein; Nutrition; Probiotics; Protein Science; Rabbits; TLR2 protein; TLR4 protein; Toll-like receptors; Tumor necrosis factor-α; Weaning; Zonula occludens-1 protein
• ISSN: 1867-1306; 1867-1314
• DOI: 10.1007/s12602-018-9476-x

Probiotics could promote animal growth and enhance immune function. This study investigated the effects of Clostridium butyricum (CB) on the growth performance, intestinal immune, and gut microbiota of weaning rex rabbits. A total of 60 healthy female rabbits (5-month-old) were divided equally into four groups and mated on the same day: control group (CTRL, fed with basal feed), low-dose group (LDG, fed with basal feed + 1.0 × 10 3  CFU/g CB), middle-dose group (MDG, fed with basal feed + 1.0 × 10 4  CFU/g CB), and high-dose group (HDG, fed with basal feed + 1.0 × 10 5  CFU/g CB). Then, 30 weaning rex rabbits (35-day-old) were collected from each group for this experiment, and they were offered the same feeds as their mother. The results demonstrated that high-dose CB treatment significantly increased average daily weight gain of weaning rex rabbits. Further studies suggested that CB enhanced small intestinal digestive enzyme activity and improved mucosal morphology and antioxidant status. Supplemented with CB, small intestinal barrier function was maintained with the upregulation of mRNA levels of ZO-1, claudin, and occludin as well as the increase of sIgA production. Moreover, the relative expressions of MyD88, TLR2, and TLR4 were elevated in HDG; simultaneously, pro-inflammatory cytokines including IL-6, INF-γ, and TNF-α were decreased after CB administration. In addition, CB showed beneficial effects in improving weaning rex rabbit intestinal microflora via increasing the abundance of beneficial bacteria. Therefore, our results indicated CB can promote rex rabbit growth, which is likely to the enhancement of immune function and the improvement of intestinal microbiota.