Characterizing the three‐dimensional organization of telomeres in papillary thyroid carcinoma cells

The relationship between the three‐dimensional (3D) nuclear telomere architecture and specific genetic alterations in papillary thyroid carcinoma (PTC), in particular in cancer stem‐like cells (CSLCs), has not yet been investigated. We isolated thyrospheres containing CSLCs from B‐CPAP, K1, and TPC‐...

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Bibliographic Details
Published in:Journal of cellular physiology 2019-04, Vol.234 (4), p.5175-5185
Main Authors: Caria, Paola, Dettori, Tinuccia, Frau, Daniela Virginia, Lichtenzstejn, Daniel, Pani, Fabiana, Vanni, Roberta, Mai, Sabine
Format: Article
Language:English
Subjects:
3D quantitative nuclear telomere analysis
Biotechnology
Cancer
cancer stem‐like cells
Cell Line, Tumor
Cell Nucleus - genetics
Cell Nucleus - ultrastructure
Fluorescence
Fluorescence in situ hybridization
Genotype
Heterogeneity
Humans
Imaging, Three-Dimensional
In Situ Hybridization, Fluorescence
Mutation
Neoplastic Stem Cells - ultrastructure
Nucleic Acid Conformation
p53 Protein
Papillary thyroid carcinoma
Phenotype
Proto-Oncogene Proteins B-raf - genetics
Proto-Oncogene Proteins c-ret - genetics
quantitative fluorescence in situ hybridization
Spheroids, Cellular
Subgroups
Telomerase
Telomere - genetics
Telomere - ultrastructure
Telomere Homeostasis
Telomere Shortening
Telomeres
Thyrocytes
Thyroid
Thyroid cancer
Thyroid Cancer, Papillary - genetics
Thyroid Cancer, Papillary - ultrastructure
Thyroid Neoplasms - genetics
Thyroid Neoplasms - ultrastructure
Tumor Suppressor Protein p53 - genetics
Tumors
Yeast
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Summary:The relationship between the three‐dimensional (3D) nuclear telomere architecture and specific genetic alterations in papillary thyroid carcinoma (PTC), in particular in cancer stem‐like cells (CSLCs), has not yet been investigated. We isolated thyrospheres containing CSLCs from B‐CPAP, K1, and TPC‐1 PTC‐derived cell lines, representative of tumors with different genetic backgrounds within the newly identified BRAFV600E‐like PTC subgroup, and used immortalized normal human thyrocytes (Nthy‐ori 3.1) as control. We performed quantitative fluorescence in situ hybridization, 3D imaging, and 3D telomere analysis using TeloView software to examine telomere dysfunction in both parental and thyrosphere cells. Among the 3D telomere profile, a wide heterogeneity was observed, except for telomere intensity. Our findings indicate that CSLCs of each cell line had longer telomeres than parental cells, according to telomere intensity values, which correlate with telomere length. Indeed, the thyrosphere cells had lower numbers of lower‐intensity telomeres (≤5,000 arbitrary fluorescent units, a.u.), compared with parental cancer cells, as well as parental control cells, (p 17,000 a.u.) than K1 and TPC‐1 cells, as well as control cells (p 
ISSN:0021-9541
1097-4652
DOI:10.1002/jcp.27321