Transcription factor-based biosensor for detection of phenylalanine and tyrosine in urine for diagnosis of phenylketonuria

Clinicians require a simple quantitative method for the detection of both phenylalanine and tyrosine to facilitate the diagnosis of phenylketonuria, a common inherited disorder of amino acid metabolism. In this study, we developed a novel whole-cell biosensor for the quantification of phenylalanine...

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Bibliographic Details
Published in:Analytica chimica acta 2018-12, Vol.1041, p.108-113
Main Authors: Lin, Chieh, Jair, Yung-Cheng, Chou, Yi-Chieh, Chen, Pai-Shan, Yeh, Yi-Chun
Format: Article
Language:English
Subjects:
Amino acids
Biosensors
coli
Diagnosis
Fluorescence
Fluorescence in situ hybridization
Medical diagnosis
Metabolism
Phenylalanine
Phenylketonuria
Proteins
Tyrosine
tyrR
Urine
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Summary:Clinicians require a simple quantitative method for the detection of both phenylalanine and tyrosine to facilitate the diagnosis of phenylketonuria, a common inherited disorder of amino acid metabolism. In this study, we developed a novel whole-cell biosensor for the quantification of phenylalanine and tyrosine through the expression of red and green fluorescent proteins. The proposed system responds specifically and sensitively to phenylalanine/tyrosine without interferences from other amino acids. Furthermore, the precision of the biosensor was evaluated using specimens of normal human urine by LC-MS. [Display omitted] •A novel cell-based approach to the analysis of phenylalanine and tyrosine in urine samples was developed.•The whole-cell biosensors exhibited great sensitivity and selectively for the detection of phenylalanine and tyrosine.•The accuracy and precision of proposed sensor for human urine samples were validated with LC-MS with satisfactory results.
ISSN:0003-2670
1873-4324
DOI:10.1016/j.aca.2018.08.053