Combined effects of osmotic and hydrostatic pressure on multilamellar lipid membranes in the presence of PEG and trehalose

We studied the interaction of lipid membranes with the disaccharide trehalose (TRH), which is known to stabilize biomembranes against various environmental stress factors. Generally, stress factors include low/high temperature, shear, osmotic and hydrostatic pressure. Small-angle X-ray-scattering wa...

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Bibliographic Details
Published in:Soft matter 2018-11, Vol.14 (43), p.8792-8802
Main Authors: Al-Ayoubi, Samy R, Schinkel, Peter K F, Berghaus, Melanie, Herzog, Marius, Winter, Roland
Format: Article
Language:English
Subjects:
Dependence
Disaccharides
Elastic properties
Environmental stress
Fluorescence
Fluorescence spectroscopy
High temperature
Hydrostatic pressure
Lipid membranes
Lipids
Low concentrations
Membranes
Osmosis
Osmotic pressure
Phospholipids
Polyethylene glycol
Thermodynamic properties
Topology
Trehalose
X ray spectra
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Summary:We studied the interaction of lipid membranes with the disaccharide trehalose (TRH), which is known to stabilize biomembranes against various environmental stress factors. Generally, stress factors include low/high temperature, shear, osmotic and hydrostatic pressure. Small-angle X-ray-scattering was applied in combination with fluorescence spectroscopy and calorimetric measurements to get insights into the influence of trehalose on the supramolecular structure, hydration level, and elastic and thermodynamic properties as well as phase behavior of the model biomembrane DMPC, covering a large region of the temperature, osmotic and hydrostatic pressure phase space. We observed distinct effects of trehalose on the topology of the lipid's supramolecular structure. Trehalose, unlike osmotic pressure induced by polyethylene glycol, leads to a decrease of lamellar order and a swelling of multilamellar vesicles, which is attributable to direct interactions between the membrane and trehalose. Our results revealed a distinct biphasic concentration dependence of the observed effects of trehalose. While trehalose intercalates between the polar head groups at low concentrations, the effects after saturation are dominated by the exclusion of trehalose from the membrane surface.
ISSN:1744-683X
1744-6848
DOI:10.1039/c8sm01343h